在大块颌骨缺损的修复治疗中，由于自体骨移植作为一种“以创伤修复创伤”的修复模式存在众多弊端，再生医学技术可望成为新的修复替代手段。然而，血管化问题一直是限制大块组织或复杂器官再生的关键因素。大块颌骨组织再生血管化问题的解决能够加快再生速度和提高再生质量；支架整体快速血管化的实现还有利于负载干细胞再生效能的发挥，进一步提高颌骨再生速度和质量。氧化石墨烯（GO）可加速干细胞的粘附和成骨分化，但具体所涉及的机制和信号通路尚且缺乏解释。我们的前期研究发现，GO-重组甲状旁腺激素（PTH 1-34）可促进骨髓间充质干细胞成骨和成血管分化。为此，本项目将围绕大块支架血管化及负载干细胞存活等问题，借助3D打印技术进行大块支架设计和制备，并涂覆GO涂层、负载PTH以协同诱导整体支架快速血管化，促进负载骨髓间充质干细胞成骨向分化，阐明ERK1/2信号通路对GO-PTH宏-微孔多级孔洞支架诱导成骨的影响。

As autologous bone transplantation exists many disadvantages, regenerative medicine technology is expected to be a new alternative method in large-size mandibular defect .However,as a a critical factor,vascularization has been limiting the regeneration of large tissues and complex organs.The revascularization of large jaw tissue can accelerate the regeneration rate and improve the quality of regeneration,and the entirely rapid vascularization of scaffolds is beneficial to the regeneration efficiency of the loaded stem cells to further improve regeneration efficiency.In our previous study ,go-recombinant parathyroid hormone (PTH 1-34) can promote bone formation and angiogenic differentiation of bone marrow mesenchymal stem cells.Therefore, we will be bent on large bracket vascularization and the survive of load cells ,design and prepare massive scaffolds by 3D printing technology ， apply the scaffolds with GO and PTH(1-34),synergistically inducing rapid vascularization of the scaffold, promoting osteogenic differentiation of the loaded bone marrow stem cells, elucidate the effect of erk1/2 signaling pathway on the osteogenesis induced by go-pth micro-porous multi-stage scaffold.