解淀粉芽孢杆菌提高植物碱胁迫抗性的机理研究

碱胁迫是植物生长的重要限制因素，对我国生态环境和农业生产形成严重威胁。已有研究证明，根部质膜H+-ATPase是植物碱胁迫响应的关键酶。我们的前期研究发现，解淀粉芽孢杆菌GB03菌株的挥发性有机物，能够影响植物根部质膜H+-ATPase活性调控基因的表达，显著提高根部质膜H+-ATPase的活性和根际酸化能力，从而提高植物碱胁迫抗性。在此基础上，本项目拟以拟南芥根部质膜H+-ATPase活性调控关键基因的突变体为研究材料，利用根部质膜H+-ATPase活性调控化合物，采取非损伤微测、免疫印迹等技术，探究解淀粉芽孢杆菌GB03的挥发性有机物调控根部质膜H+-ATPase活性、提高植物碱胁迫抗性的生理和分子机理；同时鉴定并验证GB03挥发性有机物中起调控作用的具体成分及成分组合。本项目可揭示GB03提高植物碱胁迫抗性的机理，为推动GB03及其活性成分在环境保护和农业生产上广泛应用提供理论依据。

Alkali stress is an important limiting factor for plant growth and poses a serious threat to China's ecological environment and agricultural production. Studies have shown that the root plasma membrane H+-ATPase is a key enzyme in plant response to alkali stress. Our previous study found that the volatile organic compounds of B. amyloliquefaciens GB03 strain can affect the expression of plant root plasma membrane H+-ATPase activity-regulating genes, significantly increase the activity of root plasma membrane H+-ATPase and the ability of rhizosphere acidification, thereby improving plant alkali stress resistance. On this basis, this project intends to use the mutants of the plasma membrane H+-ATPase activity regulation genes of Arabidopsis thaliana as the research material, using the root plasma membrane H+-ATPase activity regulating compounds, adopting non-invasive micro-test, immunoblotting and other techniques., to explore the physiological and molecular mechanisms by which volatile organic compounds of Bacillus amyloliquefaciens GB03 regulate the activity ofroot plasma membrane H+-ATPase and improve plant alkali stress resistance. At the same time, identify and verify the specific components and combinations of components of GB03 volatile organic compounds. This project can reveal the mechanism of GB03 to improve the resistance of plant alkali stress, and provide a theoretical basis for promoting the wide application of GB03 and its active ingredients in environmental protection and agricultural production.