糖蛋白的分离和鉴定对深入研究其生物功能、发现生物标志物及研发新型生物药具有重要意义。但是生物样品基质的复杂性和糖蛋白的丰度低导致其分离和鉴定非常具有挑战性。现有糖蛋白的富集方法存在特异性不足和非糖蛋白的非特异性吸附问题。针对这两个问题，在前期工作基础上，本项目拟仿生凝集素与糖蛋白结合时形成的多氢键体系、CH-π体系以及糖结合口袋等多因素协同作用，拟将亲水性氨基酸和疏水性芳香族氨基酸同时引入到双乳液界面聚合反应体系，制备一系列化学组成各向异性、亲疏水性质微区不同、孔径可调、拓扑结构可调的异质纳米孔材料，明确材料的形成机制，考察影响异质纳米孔材料富集糖蛋白的因素，优化和建立糖蛋白的富集方法。预实验已经证明基于异质纳米孔仿生凝集素材料设计的合理性以及在标准品层面上富集糖蛋白的可行性。此研究将为糖蛋白富集材料的设计提供新思路。

The separation and characterization of glycoproteins are important to deepen our study of the glycoproteins’ biofunctions, find out novel biomarkers and develop novel biopharmaceuticals. However, it is challenging to separate and characterize glycoproteins from biosamples because of the complexity of matrix and their low abundance of glycoproteins. The current enrichment methods for glycoproteins suffer from low selectivity and non-specific adsorption of non-glycosylated proteins. To address these issues, neolectin materials were developed inspired from specific affinity of lectins toward glycoproteins, which mainly results from the synergetic effects of multiple hydrogen bonds, CH-π system and carbohydrate-binding pocket between lectins and glycoproteins. In this proposal, hydrophilic amino acid (AA) and hydrophobic aromatic AA will be simultaneously introduced to the dual emulsion interfacial polymerization system and synthesize a series of heterostructured nanopores materials with various chemical composition, different hydrophilic/hydrophobic micro-domains, adopted pore sizes and topological structures. The formation mechanism of heterostructured nanopores materials will be clarified. The effects of experiment conditions to the enrichment of glycoproteins will be investigated. Then the enrichment method will be established and optimized. Our preliminary experiment data has proved the rationality of material design inspired by lectins and feasibility of enrichment of glycoprotein with standard samples. This study will provide new ideas for the design of glycoprotein enrichment materials.