酒精刺激BMSCs成脂能力增强、成骨能力减弱是导致股骨头坏死（ONFH）患者脂肪代谢紊乱的重要原因。内源microRNA（miRNA）在BMSCs成脂-成骨分化中起调控作用。中医学认为，“痰”和“瘀”与脂代谢紊乱密切相关。研究表明“痰瘀同治”可改善酒精性ONFH患者脂代谢异常状态，且可促兔BMSCs成骨分化。我们推测酒精性ONFH患者可能存在特异miRNA参与骨修复，且可能是祛痰逐瘀法作用的靶miRNA。本课题①拟采用高通量筛选法检测酒精性ONFH患者及祛痰逐瘀法治疗后血浆中miRNA表达谱变化，筛选差异miRNA，QPCR验证miRNA表达并预测其靶基因；②用miRNA模拟剂或抑制剂转染ONFH患者BMSCs研究差异miRNA的功能并确定其靶基因，研究祛痰逐瘀法对靶miRNA的转录调控作用；③研究祛痰逐瘀方联合最差异表达miRNA调控的靶基因转染BMSCs移植修复兔酒精性ONFH的效果。

Alcohol enhanced ability to stimulate BMSCs adipogenic, osteogenic diminished capacity is an important cause of osteonecrosis (ONFH) in patients with disorders of fat metabolism. Endogenous microRNA (miRNA) in BMSCs into adipocytes - from regulation of osteogenic differentiation. Chinese medicine believes that "phlegm" and "stasis" is closely related to lipid metabolism. Studies have shown that "Phlegm" may improve lipid metabolism in patients with alcoholic ONFH abnormal state, and can promote osteogenic differentiation of rabbit BMSCs. We speculate that there may be alcohol ONFH patient-specific miRNA involved in bone repair, and may be expectorant action Stasis target miRNA. This paper intends to use after treatment ① high-throughput screening assay ONFH alcoholic patients and expectorant stasis law changes in plasma miRNA expression profiling, screening differences in miRNA, QPCR validated miRNA target gene expression and predict; ② with miRNA mimetic or inhibitors in patients with BMSCs transfected ONFH differences of miRNA function and determine its target genes, transcriptional regulation of research expectorant stasis method of miRNA targets; ③ joint research expectorant stasis party most differentially expressed miRNA regulation of target gene transfection repair of rabbit BMSCs transplantation in alcoholic ONFH.