链格孢菌是引起葡萄采后病害的主要病菌，其产生的真菌毒素对人体健康有巨大危害。前期研究发现肉桂醛可抑制链格孢菌生长，且H2O2可能参与调控肉桂醛对非寄主选择性毒素合成的影响。本项目主要研究：1）测定肉桂醛、肉桂醛+H2O2和肉桂醛+DMTU对非寄主选择性毒素合成的影响；2）利用RNA-seq和iTRAQ技术筛选不同处理间的差异基因、转录因子及受影响的代谢途径；3）利用荧光定量PCR验证基因表达并明确受影响的代谢途径，分析差异基因启动子序列，确定转录因子作用元件，从分子生物学的角度确定H2O2参与调控链格孢菌非寄主选择性毒素合成的方式；4）测定受影响代谢途径中的酶活性，从生理生化角度验证参与肉桂醛介导H2O2影响非寄主选择性毒素合成过程的代谢途径。研究结果从生理生化及分子生物学角度阐明肉桂醛介导H2O2对链格孢菌非寄主特异性毒素合成的双向调控机制，可为控制采后葡萄果实真菌毒素污染奠定理论基础。

Alternaria alternata is one of major fungus causing the decay of mildew rot in grape fruits, it could generate mycotoxins during the fungi growth and result the harm to human health. Cinnamaldehyde, a kind of plant essential oils, which proved to be effectively against A. alternata, but further investigation on the regulations of mycotoxins biosynthesis was remain undetermined. This study will start with the effect of cinnamaldehyde on the inhibition of A. alternata mycotoxins biosynthesis, mainly focus on: 1) the optimization of HPLC for mycotoxins measurements, and will study the effect of cinnamaldehyde, cinnamaldehyde + H2O2, and cinnamaldehyde + DMTU treatment on the synthesis of Non-Host Specific Toxin (NHST) in A. alternata at different culturing time; 2) the transcriptome sequencing (RNA-seq) will be adapted to reveal the transcriptome profilings of A.alternata after different treatments; 3) the isobaric tags for relative and absolute quantitation (iTRAQ) techniques will be used for analysing the proteome profilings after A. alternata cultured with cinnamaldehyde, H2O2 and DMTU. 4) the quantitative real-time PCR will be conducted to confirm the correlation analysis results of transcriptome and proteome profilings, thereby to reveal the acting elements of transcription factor in the promoter of differential genes, and the molecular regulatory mechanism of H2O2 on the synthesis of Non-Host Specific Toxin. 5) will investigate the changeable enzymatic activity in the differential metabolic pathway (mainly focus on REDOX metabolic pathway), finally to reveal the bi-directional control mechanism of H2O2 on the synthesis of Non-Host Specific Toxin in Alternaria spp. treated Cinnamaldehyde. This study will interpret the regulation mechanism of NHST synthesis from physiology, biochemistry and molecular biology level after cinnamaldehyde, H2O2 and DMTU treatments, and the results of the study will provide a solid theoretical foundation on the effect of essential oils on mycotoxins synthesis.