番茄Mi-1基因介导的根结线虫抗性伴随着Ca2+、ROS、MPK级联反应以及HR反应等，而根系温度升高对根结线虫的抗性具有抑制作用，但具体的机制仍不清楚。申请者近年来研究发现，细胞氧化还原（redox）信号与植物的抗性具有重要的联系，并且温度对redox的调控可能与植物的温度适应性有关，因而提出根系高温环境下细胞redox状态趋于氧化，阻断了SA等抗病信号通路，继而抑制根结线虫抗性。本项目拟以不同温敏性抗根结线虫番茄品种为材料，利用qRT-PCR、基因表达谱和VIGS等技术，阐明redox信号在不同温度下对根结线虫抗性的作用。研究抗病复合体蛋白组分如HSP90水平、亚细胞定位和抗病热稳定性之间的相互关系，同时，希望利用BR和SA等调控手段提高温敏性抗性番茄的根结线虫抗性，并研究其生理与分子机制。研究有望为抗病基因资源的高效利用和根结线虫的抗性调控提供理论与实践基础。

Mi-1gene-mediated resistance to nematode is accompanied by changes of Ca2+, ROS, MPK cascade and HR response in tomato. The resistance is suppressed by high root temperature; however, the mechanism is still unclear. In recent years, the applicant has discovered that cellular redox state is critical for the induction of stress tolerance and acclimation for the temperature of the habitat of the plant species. Therefore, the applicant proposes the hypothesis that the oxidized cellular redox state is attributable for the impaired SA signaling and inhibition of nematode resistance under high temperature. By using qRT-PCR, gene profiling, and VIGS, the project is aimed at elucidating the role of redox signaling in temperature-dependent nematode resistance. The project will also study changes of the content and subcellular localization of components of defence protein complex and the relationship with thermo-stability of nematode resistance. Finally, the project plans to regulate the nematode resistance of tomato by applying BR and SA, and meanwhile, understand the physiological and molecular mechanisms underlying these effects. The project is expected to provide theoretic and practical bases for the efficient use of resources of resistance genes and the chemical regulation of nematode resistance.