喉鳞癌是常见的头颈恶性肿瘤，其发病机制一直未被阐明，而喉癌干细胞被认为是喉癌发生、发展和放化疗耐受的根源。近年来原癌基因BCL11A在肿瘤发生过程中的作用开始得到人们的重视。但与喉癌的关系还不明确，我们前期发现，BCL11A是促进喉癌的危险因素，且其高表达与局部复发、死亡高度相关联；BCL11A上调了喉鳞癌中干细胞细胞的比例而且增大了顺铂化疗的耐药性。本课题拟通过对喉鳞癌患者外周血、肿瘤组织、细胞系以及喉癌荷瘤鼠肿瘤模型进行喉癌干细胞的化疗耐药相关研究，通过干预BCL11A的表达来研究喉鳞癌干细胞的特性变化与化疗耐药性的联系，此外采用RNA-seq等方法探索BCL11A调控喉癌干细胞化疗耐药的靶点。通过上述研究，本项目试图阐明BCL11A调控喉癌干细胞参与化疗耐药的分子机制，寻找提高喉癌化疗敏感性的新方法。

Laryngeal squamous cell carcinoma is one of common head and neck tumors and its pathogenesis has not been able to clarify. Laryngeal stem carcinoma cell is considered the source of laryngeal cancer occurrence, development and radiotherapy and chemotherapy resistance. More attention has been attracted for the role of BCL11A in the tumorigenesis process. Our preliminary studies have found that BCL11A is a risk factor for laryngeal cancer，what is more，its high expression is associated with local recurrence and survival rate. Further, BCL11A upregulated the proportion of laryngeal squamous cell carcinoma stem cells and increased the chemotherapy drug resistance. Therefore, the project intends to conduct chemoresistance of laryngeal carcinoma stem cell research by laryngeal squamous cell carcinoma tumor tissue, peripheral blood, cell lines and tumor models laryngeal tumor-bearing mice, meanwhile, To investigate the relationship between changes of laryngeal squamous cell carcinoma stem cells and chemoresistance by interfering with the expression of BCL11A, so as to explore the potential therapeutic target of BCL11A regulation of laryngeal cancer stem cell resistance to chemotherapy. Through this project, this issue attempts to elucidate the molecular mechanism of BCL11A regulating laryngeal cancer stem cell chemoresistance and to find new ways to improve the chemosensitivity of laryngeal cancer.