叶片是植物光合作用的重要器官，叶片发育机制的研究是农作物株型分子改良的基础。在拟南芥等模式植物中对叶片极性发育的基因调控机理已有较为深入的探讨，但是在单子叶植物尤其在水稻中这方面的研究还相对薄弱。水稻是我国重要的粮食作物，适度卷曲的叶片形态是水稻超高产理想株型的重要性状。本研究水稻卷叶突变体drl来源于籼型恢复系缙恢10号的EMS诱变体库。突变体drl叶片全生育期筒状卷曲至成熟期变窄，植株矮化。组织细胞学观察表明突变体叶片部分小维管束的远轴面厚壁细胞被叶肉细胞取代；维管系统发育异常；叶片的近远轴极性发育异常，叶片远轴面大瘤状乳突组织消失，而近轴面却出现了大瘤状乳突组织。遗传分析表明drl突变性状受隐性单基因控制，已将突变基因定位在第3染色体长臂端粒约95.3kb区域内，在该区域内还未见相似突变体报道。本研究拟图位克隆DRL基因，并利用分子生物学、蛋白质组学等技术研究其功能和作用机理。

Leaf is the major organ for photosynthesis, research for leaf development mechanism is the basis for molecular improvement of crop plant type. The study of regulatory mechanism on the development of leaf polarity is particular in model plant Arabidopsis, However, it remains to be elucidated in monocots, especially in rice. Rice is the main staple food for a large segment of the world population. Moderate leaf rolling is an important traits of ideal plant morphology in high-yield breeding. In this study, we identified a dwarf and rolling leaf mutant drl from an ethyl methane sulfonate（EMS）mutagenesis Library of indica restorer variety Jinhui 10, which exhibited phenotypes of extremely rolled leaves during all of the stages and narrowed leaves and dwarfism which appeared during the mature stage. Histological observations indicate that drl does not form abaxial sclerenchymatous cells in a portion of small vascular bundles and replaced by mesophyll cells; the development of vascular system and the polar of adaxial-abaxial is abnormal; Abaxial surface of leaves lacking the papillaes and it can be observed at the adaxial surface. Genetic analysis showed that the drl is controlled by a single recessive gene, the DRL gene was initially localized within an interval of 95.3kb physical distance on the telomere of long arm on chromosome 3. In this study, we intend to Map-based cloning of the DRL gene and study the function and the mechanism by using molecular biology and proteomics technologies.