茉莉酸信号通路介导的寄主防卫反应，是番茄果实抵御灰霉病菌侵染的主要途径；但该信号通路随miR156含量衰减而逐渐失敏，相关机制目前尚不清楚。本项目针对JA途径关键抑制因子JAZs泛素化降解是否受到miR156衰减过程抑制这一科学问题，利用蛋白质组学、免疫印迹技、免疫共沉淀与Y2H技术，探索不同成熟体系中JA信号源对JAZs泛素化过程的调控能力以及miR156靶蛋白SPLs的累积趋势，研究SPL与JAZ蛋白互作及其阻遏JAZ泛素化降解的分子机制，进而从表观遗传学层面阐明番茄果实抗灰霉病衰退的分子机理。同时，利用代谢组学方法探索果实次生代谢产物差异性响应，解析果实糖代谢对miR156的调控效应，构建miR156与JAZs动态负调控抗病基因网络模型，探索果实糖代谢→miR156→JAZs→抗病物质→腐败危险系数生化标记体系，为绿色、现代化果蔬保鲜体系提供理论依据。

The defence response mediated by JA signal is the main weapon against Botrytis cinerea infection in tomato, while the signal pathway is gradually suppressed with the decrease of the content of miR156. The association between the decline of miR156 and the ubiquitination degradation process of protein containing Jasmonate ZIM-domain (JAZ) is the key point of the JA signal pathway blocking. In the present project, iTRAQ-based quantitative proteomics and Western blotting were used to research the response ability of SlJAZ family proteins for extra-MeJA treatment in tomato at different mature degree, and the trend of miR156-target protein SlSPLs accumulation. Meanwhile, exploring mechanism behind SlSPL interacts with SlJAZ, and the SlSPL represses SlJAZ ubiquitination in tomato fruit with the method of Co-immunoprecipitation and Y2H technology. What's more, we explore the differences of secondary metabolites during fruit growth and maturation, and the regulatory effect of fruit sugar metabolism on miR156, as a verification mechanism for correlation analysis to construct the dynamic gene network regulated by miR156 and SlJAZs. In conclusion, the present results were aimed to clarify the molecular mechanism behind the decline of disease resistance to Botrytis cinerea in tomato fruit, developing the biochemical marker system of sugar metabolism-miR156-JAZs-rot risk factors, provides a theoretical basis for green and modern fruit and vegetable preservation system.