Asxl1在根尖周炎骨破坏中的调控作用

根尖周炎是指发生于根尖周组织的炎症性疾病，是口腔的常见病，其典型病理特征是根尖周骨破坏。慢性根尖周炎造成的骨破坏是由宿主免疫介导，对细菌和内毒素产生的炎症性反应，而炎症性反应造成骨质破坏的机制较为复杂，仍有待研究。Asxl1基因与骨代谢关系密切，该基因缺失可导致多种骨骼发育缺陷。了解Asxl1在炎症性反应造成骨质破坏中的调控作用，可为根尖周炎骨破坏的防治提供可能的靶向基因。本课题拟通过控制Asxl1在体外培养细胞中的表达水平，研究该基因对成骨及破骨细胞分化的影响；在此基础上应用LPS刺激细胞，观察Asxl1表达变化情况，探讨该基因在炎性微环境影响骨代谢中的作用；同时构建大鼠根尖周炎及Asxl1沉默和过表达模型，通过在体研究进一步明确Asxl1对根尖周炎骨破坏的调控。本项目将丰富根尖周骨代谢以及根尖周炎导致骨破坏的相关理论，为制定根尖周炎骨破坏的防治计划及研制促根尖周骨质生成材料提供思路。

Apical periodontitis is an inflammatory disease which happening to periapical tissues. It's a common and frequently occurring oral disease. The typical pathologic and clinical feature is periapical bone destruction. The bone loss is a host inflammatory response to bacteria and endotoxin. The mechanism of inflammatory response causing bone loss is complicated and remains to be studied. Asxl1 gene is closely associated with bone metabolism and its deletion can lead to a variety of bone defects. Understand the regulation of Asxl1 in inflammatory reaction causing bone loss can provide possible target genes for prophylaxis and treatment. The present study is proposed to explore the effects of Asxl1 on bone formation and bone resorption through control the expression of Asxl1 and detection of osteogenesis and osteoclast differentiation. And on this basis we explore Asxl1 role in inflammatory microenvironment affect bone metabolism through stimulating cells with LPS and observing the expression of Asxl1. To further illustrate Asxl1 regulation in apical periodontitis bone loss in vivo, apical periodontitis model in rats were established. The results of this project would enrich theories of periapical tissue metabolism as well as apical periodontitis causing bone loss, and provide new thought for drawing up the prevention and treatment strategies of bone destruction and developing materials for promoting bone regeneration.