柚皮苷经SDF-1/CXCR4轴介导内皮祖细胞促血管新生促进骨质疏松性骨折愈合的研究

最新研究证实由成骨细胞、破骨细胞及血管新生参与的“三元机制”，在调控骨的代谢与修复过程中发挥着更重要的作用。促血管新生已经成为治疗骨折的重要策略。已有的研究证实柚皮苷具有显著抗骨质疏松作用，并能促进血管新生。SDF-1/CXCR4轴是血管系统中调控血管新生的重要机制，因此我们提出假说：柚皮苷基于SDF-1/CXCR4轴介导内皮祖细胞（EPCs）促血管新生，进而促进骨质疏松性骨折的愈合。采用原代大鼠EPCs细胞，观察柚皮苷对EPCs细胞增值、迁移、成血管的影响；建立骨质疏松性骨折模型，观察骨质疏松性骨折与普通骨折骨痂中血管新生的差异，并采用激光多普勒、Micro-fil灌注血管成像等技术观察柚皮苷对骨质疏松性骨折新生血管的影响，探讨柚皮苷对EPCs动员、归巢及参与骨质疏松性骨折愈合过程中血管新生的作用及其机制，为柚皮苷促进骨质疏松性骨折愈合提供新的理论基础。

The latest study confirms metabolism and bone repair process by osteoblasts, osteoclasts and angiogenesis "Three mechanism" regulation. Angiogenesis has become an important strategy for the treatment of osteoporotic fractures. A large number of preliminary experiments observed that naringin significant anti-osteoporosis and promote angiogenesis. SDF-1/CXCR4 axis is an important mechanism for regulating angiogenesis. Therefore, we propose the hypothesis that naringin based on SDF-1/CXCR4 axis-mediated endothelial progenitor cells (EPCs) to promote angiogenesis for osteoporotic fracture. In vitro, EPCs isolated form rats were observed for the effect of naringin on cell proliferation, migration, and apoptosis in a blood vessel. In vivo, we observed the effect of naringin on osteoporotic fracture neovascularization using laser Doppler, Micro-fill vascular perfusion imaging; explore the effect of naringin on EPCs mobilization, homing and the role of vascular freshmen. Which could provide new treatment ideas support for EPCs for osteoporotic fracture healing, and reveal the mechanism of naringin treatment of osteoporotic fractures.