Naringin-inlaid silk fibroin/hydroxyapatite scaffold enhances human umbilical cord-derived mesenchymal stem cell-based bone regeneration.
Naringin-inlaid silk fibroin/hydroxyapatite scaffold enhances human umbilical cord-derived mesenchymal stem cell-based bone regeneration.
复制标题
柚皮苷镶嵌丝素蛋白/羟基磷灰石支架增强人脐带间充质干细胞骨再生
DOI：
10.1111/cpr.13043
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发表时间：
2021-07
影响因子：
8.5
通讯作者：
Sun L
中科院分区：
文献类型：
--
作者：
Zhao ZH;Ma XL;Zhao B;Tian P;Ma JX;Kang JY;Zhang Y;Guo Y;Sun L
巨大的骨缺陷是一种常见的、使人衰弱的临床疾病，给全球健康和经济带来巨大的负担。骨组织工程技术已成为修复缺损骨的最有前景的方法之一。在本研究中，我们制备了一种柚皮苷嵌入型复合丝素蛋白/羟基磷灰石(NG/SF/HAP)支架，用于修复骨缺损。采用盐析法制备了NG/SF/HAP支架。通过扫描电子显微镜、活/死细胞染色和鬼臼糖苷染色评价NG/SF/HAP支架的细胞相容性。体外和体内评价其成骨和血管生成特性。NG/SF/HAP多孔支架具有良好的仿生多孔结构，具有诱导成骨和血管生成的活性。基因芯片鉴定了在SF-nHAp支架上培养的人脐带间充质干细胞(HUCMSCs)和在NG/SF/HAP支架上培养的细胞之间的854个差异表达基因。利用体外培养的hUCMSCs研究其成骨机制。柚皮苷可促进hUCMSC向SF/HAP支架内生长，促进成骨分化。在NG/SF/HAP支架中培养的细胞的成骨和血管生成能力优于在SF/HAP支架中培养的细胞。这些数据显示了含有柚皮苷的SF/HAP复合支架在骨再生方面的潜力。总体而言，柚皮苷镶嵌的丝素蛋白/羟基磷灰石支架可增强基于人脐带间充质干细胞的骨再生。
Large bone defects are a common, debilitating clinical condition that have substantial global health and economic burden. Bone tissue engineering technology has become one of the most promising approaches for regenerating defective bones. In this study, we fabricated a naringin‐inlaid composite silk fibroin/hydroxyapatite (NG/SF/HAp) scaffold to repair bone defects. The salt‐leaching technology was used to fabricate the NG/SF/HAp scaffold. The cytocompatibility of the NG/SF/HAp scaffold was assessed using scanning electron microscopy, live/dead cell staining and phalloidin staining. The osteogenic and angiogenic properties were assessed in vitro and in vivo. The porous NG/SF/HAp scaffold had a well‐designed biomimetic porous structure with osteoinductive and angiogenic activities. A gene microarray identified 854 differentially expressed genes between human umbilical cord‐derived mesenchymal stem cells (hUCMSCs) cultured on SF‐nHAp scaffolds and cells cultured on NG/SF/HAp scaffolds. The underlying osteoblastic mechanism was investigated using hUCMSCs in vitro. Naringin facilitated hUCMSC ingrowth into the SF/HAp scaffold and promoted osteogenic differentiation. The osteogenic and angiogenic capabilities of cells cultured in the NG/SF/HAp scaffold were superior to those of cells cultured in the SF/HAp scaffold. The data indicate the potential of the SF/HAp composite scaffold incorporating naringin for bone regeneration. Overall schematic illustration of naringin‐inlaid silk fibroin/hydroxyapatite scaffold enhances human umbilical cord‐derived mesenchymal stem cell‐based bone regeneration.
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影响因子：
9.8
作者：
Filipowska J;Tomaszewski KA;Niedźwiedzki Ł;Walocha JA;Niedźwiedzki T
通讯作者：
Niedźwiedzki T
影响因子：
3.3
作者：
Chen, Kuo-Yu;Chung, Chia-Mei;Yao, Chun-Hsu
通讯作者：
Yao, Chun-Hsu
影响因子：
5.4
作者：
Maisani, Mathieu;Sindhu, K. R.;Chassande, Olivier
通讯作者：
Chassande, Olivier
影响因子：
8.2
作者：
Kim, Seung Hyun L.;Lee, Seunghun S.;Hwang, Nathaniel S.
通讯作者：
Hwang, Nathaniel S.
用于血管化骨再生的共同启发的基于羟基磷灰石的支架
影响因子：
9.7
作者：
Feng, Chun;Xue, Jianmin;Wu, Chengtie
通讯作者：
Wu, Chengtie