年龄相关性黄斑变性是目前常见的与年龄相关的不可逆致盲性眼病,全基因组关联分析发现年龄相关性黄斑病变易感因子2(age-related maculopathy susceptibility2，ARMS2)和高温必需因子A-1 (high-temperature requirement factor A-1，HTRA-1)等基因是AMD发生的高危因素，而这些易感基因的作用机制尚不明确，不同基因型的患者对眼部营养药物及抗氧化药物的反应亦存在差异，个体化AMD致病机制研究及治疗势在必行。目前缺乏多基因实验模型限制了研究的发展，我们利用诱导多能干细胞技术，根据RPE细胞生理特点，加入外源性脂褐素荧光基团化合物A2E，老化ARMS2/HTRA1易感基因患者自体iPS-RPE细胞，探索最佳老化条件，明确细胞表型，为自体AMD细胞模型的建立、发病机制的研究及未来个体化药物治疗开辟新的思路。

Age-related macular degeneration (AMD) is one of the most common reasons leading to unreservable blindness of aged people. Although the closely linked genome-wide association studies (GWAS) ARMS2/HTRA1 genes, are strongly associated with the risk of AMD, their downstream targets are unknown, studying how these risk alleles lead to pathology remains a challenge. Moreover, the effect of the same medicine on different allies are variable. Induced pluripotent stem (iPS) cell-derived RPE from patients provides us with earlier stage AMD patient-specific cells and allows us to analyze the underlying mechanisms at this critical time point. We aged iPS-RPE cells by lipofusion components A2E treatment based on the biological character of RPE cells, to explore the best dose of A2E for aging cells, to compare the different phenotype of A2E-aged patient-specific iPS-derived RPE cell lines from wide-type iPS-derived RPE cell lines by unbiased proteome screen.