利用植物源杀虫活性物质通过“推-拉”策略防治害虫是目前国内外害虫生物防治的研究热点之一。然而，因受限于害螨基因组学信息的匮乏，特别是嗅觉相关基因信息，致使该领域分子机制的研究甚少。本研究在前期研究的基础上，通过全长转录组测序分析，鉴定朱砂叶螨气味结合蛋白（OBPs）编码基因，利用荧光定量PCR技术检测植物源杀螨活性物油酸乙酯（EO）对其基因表达量的影响；通过荧光竞争结合试验，探究EO与朱砂叶螨OBPs的结合能力，筛选EO可能作用靶点蛋白；采用同源建模与分子对接技术模拟分析该蛋白与EO的识别与结合特性，预测其关键结合位点；并通过蛋白免疫印迹和免疫组化技术对该靶点蛋白进行免疫组织定位。预期研究结果不仅能有助于深入理解活性物质对害螨的驱避机制，还可为挖掘有效新作用靶点以及实现该化合物的产业化和商品化提供技术与理论支撑。

The use of push-pull strategies via the active compounds from plants in integrated pest management has been the object of considerable global research interest and become an important direction for research. However, due to the limit of genome information of mites, especially of the olfactory, the molecular mechanism in this field is scarcely explored. Based on the previous work, this study will be conducted to analyze and identify the odorant binding proteins (OBPs) genes from Tetranychus cinnabarinus by full-length transcriptome sequencing, and detect the expression differences of OBPs genes when T. cinnabarinus population were treated with ethyl oleate (EO) by qPCR. Through fluorescence competition test, the potential target protein of EO which has strong binding ability with EO will be screened out. To investigate the binding characteristics of this protein and EO, homology modeling and molecular docking techniques are performed and the key binding sites also can be predicted. In addition, the distribution of the potential target protein will be explored by immunohistochemistry analysis and Western blotting. The expected results will not only contribute to deeply understanding the molecular mechanism of mite repellents, but also provide the basis for exploring some new targets and realizing the industrialization and commercialization of this compound.