花序的形态建成对水稻产量形成至关重要，目前人们对类受体激酶介导的跨膜信号转导在花序发育调控中的作用研究得很少。我们最近报道了两个类受体激酶DRUS1和DRUS2功能冗余地调控水稻生殖发育过程，它们通过调控花序分生组织的活力影响花序的形态建成。然而，DRUS1调控花序发育的信号通路并不清楚。我们前期通过免疫共沉淀结合质谱测序技术以及酵母双杂交的初步验证，发现了DRUS1的候选互作蛋白OsYODA1，一个丝裂原活化蛋白激酶（MAPKKK）。本项目将通过一系列遗传和生化手段，研究OsYODA1在DRUS1介导的调控花序发育信号通路中的作用，并确定OsYODA1的信号级联磷酸化元件及下游调控靶基因，以期解析OsYODA1作为DRUS1介导的跨膜信号通路下游组分参与调控花序发育的分子机制。

The inflorescence morphogenesis is the crucial determinant for grains formation and yield potential in rice. So far, the essential roles of receptor-like kinases in mediating transmembrane signal transduction to control the inflorescence development has been rarely reported. Recently, we have reported that two receptor-like kinases DRUS1 and DRUS2 redundantly control the reproduction development in rice. They promote the inflorescence morphogenesis by activating the inflorescence meristem viability. To elucidate the DRUS1-mediated signal pathway in the inflorescence development, we have identified a putative DRUS1-interacting protein, OsYODA1, a mitogen-activated protein kinase (MAPKKK), using co-immunoprecipitation combined with mass spectrometry analysis. Their interaction had been confirmed by yeast-two-hybrid assay. In this project, we will study the roles of OsYODA1 in the inflorescence and uncover a MAPKKK phosphorylation cascade module and its downstream target gene. We will investigate the molecular mechanism of OsYODA1, as a DRUS1 downstream signaling component in the regulation of inflorescence development.