水稻的花序发育与产量密切相关，研究调控花序发育的基因具有重要的理论和现实意义。前期工作表明，两个编码CrRLK1L类受体激酶的基因DIS1和DIS2同时降低表达后，水稻呈现花序短小稀疏的表型。CrRLK1L类受体激酶在单子叶植物水稻中的功能未见报道。本项目首先通过观察DIS1和DIS2双重突变体dis1-1 dis2，明确它们功能冗余地调控水稻花序的发育。据报道，N-连糖基化修饰对膜蛋白的分选和功能都很重要，但CrRLK1L类受体激酶胞外域N-连糖基化修饰的功能未知，本项目将以DIS1和DIS2的双重突变体dis1-1 dis2的花序发育为背景材料，通过遗传转化将不同N-连糖基化位点突变的DIS1和DIS2回转双突，重点解析胞外N-连糖基化修饰对于CrRLK1L类受体激酶功能的重要性，找到对DIS1和DIS2调控花序发育贡献最大的关键位点，为探索DIS1和DIS2的胞外信号分子奠定基础。

The rice inflorescence development is directly related with the production, so it’s significant in theory and reality to study the functional genes controlling this developmental progress. The previous works showed that knocking-down of two CrRLK1L receptor-like kinase coding genes DIS1 and DIS2 resulted in short and sparse inflorescence. The function of CrRLK1L receptor-like kinases in rice is poorly understood. Here we will firstly confirm that DIS1 and DIS2 redundantly control the inflorescence development in rice through observing the phenotypes of their single and double mutants. N-linked glycosylation is important for the selection of secretary and membrane proteins, we found nine N-linked glycosylation sites on the extracellular region of DIS1 and DIS2, respectively, and will secondly analyze the importance of these N-linked glycosylations for their functions. Through transforming different form of single N-linked glycosylation site mutant DIS1 and DIS2 into dis1-1 dis2 double mutant, we will find the key sites essential for the function of DIS1 and DIS2 in regulating inflorescence development, and that will provide information for their extracellular signaling molecules.