Toll样受体介导的中性粒细胞胞外陷阱在输卵管感染中的作用研究

近年来输卵管感染的发病率居高不下，直接危害着人类的生殖健康，如何有效地治疗并防止其后遗症的发生是期待解决的问题。中性粒细胞胞外陷阱（NETs）杀菌机制具有高效、广谱、低能耗、低组织损伤的特点，成为抗感染免疫学研究的热点。本项目拟在前期研究的基础上从降低炎症对组织损伤为切入点对Toll样受体、NETs、糖皮质激素与输卵管感染进行深入研究。确定TLRs可以诱导中性粒细胞产生NETs；通过离体的感染模型、输卵管细胞培养和在体输卵管感染的研究，从理论上阐明了输卵管炎症时GCs、TLRs、NC、NETs之间的相互关系及其作用， 上述结果可能为生殖道天然免疫机制提出新的学术观点，并可为深入了解输卵管炎症的抗感染机制以及开发治疗药物的靶点提供新的思路,有助于急慢性输卵管炎症的治疗策略的开发，改善生育结局。

In recent years increasing incidence of tubal infection directly hazards on human reproductive health. How to effectively treat and prevent its sequelae is becoming more and more important theme in the medicine. The neutrophil extracellular traps (NETs) trap and kill bacteria in high efficiency, broad-spectrum bactericidal mechanism with low energy consumption, minimal damage to host cells, also as the hot spot in now immunological domain. With the original purpose to therapy the tubal infection effectively and low tissue damage, we focused on the possible relation between toll-like receptors (TLRs), glucocorticoid, NETs and tubal infections in this study. The toll-like receptors and NETs were detected by the agent stimulating test, blocking TLRs test, also its role in the human fallopian tube. On the parallel glucocorticoid was explored whether it can therapy also protect the inflamed fallopian tube through in-vitro and in-vivo tubal infection and cultured tubal cell line. All above consequence would contribute further to current knowledge of mechanisms involved in innate immunity of female reproductive tract; also offer original idea for thorough comprehension of anti-infection mechanism during salpingitis then exploitation the target of its medicine therapy, thereby beneficial developing strategy of acute or chronic salpingitis therapy and amendment the human production.