基于“体热平衡”原理探讨芍药甘草汤“酸甘化阴”配伍抵御高温环境大鼠死亡的机理

高温作业损害是当前从事农业、军事、建筑等众多行业群体不可回避的现实问题。芍药甘草汤是仲景酸甘化阴配伍的代表方剂，原方主治“脚挛急”的症候表现与高温作业之“热痉挛”病有诸多相似之处。课题前期研究发现：芍药甘草汤可显著抵御高温环境（36±1℃）大鼠死亡，提高大鼠骨骼肌PGC1α、CS、OGDH活性，增加大鼠出汗量，故课题组提出“芍药甘草汤抵御高温环境大鼠死亡机理与调控‘体热平衡’有关”的假说，拟通过分析该方对正常大鼠、中枢神经麻醉模型大鼠“产热”环节T3、T4、TSH、TRH、CS、OGDH、线粒体RCR、COXⅣ等及“散热”环节Na+、K+、Ca2+、Mg2+、Ach、E/A、M3受体、β2受体等的影响，明确该方抵御高温与调控“体热平衡”的关系，阐明其“酸甘化阴”配伍原理的内涵，为临床应用该方治疗“脚挛急”证、预防或降低高温对机体的损害、扩大其临床用途、开发抗高温保健品或新药奠定实验基础。

High temperature operation damage is a practical problem that can not be avoided by people engaged in agriculture, military, architecture and many other industry groups.Created by Zhang Zhongjing,the Shaoyao Gancao Decoction is a representative formula to dissect “Suangan Huayin”theory.And the Symptoms of “Cramp Foot”,an ancient Chinese disease that can be cured by this formula,resemble those of the high-temperature-operation-caused “Heat cramps”.Previous studies showed Shaoyao Gancao Decoction can significantly resist death of rats in high temperature environment（36±1℃）, increase the activity of PGC1 α, CS, OGDH in rat skeletal muscle and increase the amount of sweating.So the research group hypothesises that mechanism of Shaoyao Gancao Decoction resists rats' death in high temperature environment is related to the regulation of body heat balance.By analyzing the influence of the decoction to the heat production receptor T3, T4, TSH,TRH, CS, OGDH, mitochondrion RCR and COX IV as well as radiating receptor Na+、K+、Ca2+、Mg2+、Ach、E/A、receptor M3 and β2 of both normal rats and Central nervous narcotic rats,we are abled to identify the relationship in how this formula resist high temperature and regulate body heat balance.Also,the theory of “Suangan Huayin” is clarified to provide experimental basis for the clinical application of treating “Cramp Foot”,the prevention or damage reduction of high temperature,the expansion of clinical use and the development of new drugs or health care products.