基于线粒体能量代谢动态变化探讨桂枝甘草汤"辛甘化阳"配伍原理

桂枝甘草汤是"辛甘化阳"配伍的代表方剂，但其配伍原理的现代内涵依然不够清晰。"辛甘"配伍为什么能"化阳"？"化"的机理和"阳"的本质究竟是什么？是否与线粒体能量代谢有关？本课题以"生命动力"为切入点，将中医"阳气"和现代医学"能量"联系起来，提出"阳气化生与能量代谢动态相关"假说。通过观察桂枝甘草汤及其拆方对正常动物、模型动物在常温、低温、高温环境下给药第5、10、15天体重、体温、摄食量；血清GLU、T3、Insulin、GC、TC、TG、Urea含量； 骨骼肌与心肌CS、α-KGDHC、PGC-1α活性，COXⅣ、ATP5b mRNA表达水平，AMP、ADP、ATP含量，线粒体ADP/O、RCR、形态等能量代谢相关指标的动态影响，揭示桂枝甘草汤"辛甘化阳"配伍原理与线粒体能量代谢的关系，为方剂性味配伍原理的内涵研究提供新的思路，为指导临床合理运用桂枝甘草汤治疗"心阳虚证"提供实验依据。

Guizhi Gancao Decoction is a representative prescription of "XinGan HuaYang"compatibility. But the modern connotation of its compatibility principle is still not clear. Why does"XinGan" compatibility can "HuaYang"？What is the mechanism of "Hua" and the nature of "Yang"？Whether it is associated with mitochondrial energy metabolism? This project discusses the "life vitality" as the entry point, connects the "Yang" of Chinese medicine with the "Energy" of modern medical science and puts forward the hypothesis of "the dynamic correlation of Yang and Energy". The Decoction of Cassia trig and Liquorice and its broken prescriptions are used to observe the weight、temperature and food consumption of normal animals and model animals in normal、low and hot environment, In the administration of 5, 10 and 15 days ，as well as the Serum GLU、T3、Insulin、GC、TC、TG、Urea's content and The Skeletal and cardiac muscle's CS、 α-KGDHC、PGC-1α's liveness, COXⅣ、ATP5b mRNA's expression levels, AMP、ADP、ATP's content and the dynamic changes of relevant indexes of energy metabolism like mitochondrion ADP/O、RCR and forms，to reveal the principle of medicine compatibility of Guizhi Gancao Decoction's XinGan HuaYang and mitochondrial energy metabolism relationship，and with the purpose of providing new thought train for the research of the principle of the compatibility of TCM prescriptions' features and tastes and provide evidence for properly guiding clinical practice.