化疗药物作为治疗肿瘤的常用方法，是导致女性肿瘤患者生育能力下降的主要原因。人羊膜上皮细胞移植具有修复化疗后卵巢功能的作用，主要表现为抑制化疗诱导的颗粒细胞凋亡及促进卵泡发育与成熟。前期研究发现，其旁分泌途径在其修复卵巢功能过程中发挥重要作用，但具体分子机制并不清楚。外泌体是一种细胞与细胞之间交流的重要介质，干细胞旁分泌途径中的重要组成部分。干细胞分泌的外泌体通过传递特定的microRNAs调控受体细胞的功能并参与组织损伤的修复过程。在本研究中，首先分析人羊膜上皮细胞来源外泌体的生物学功能以及其对化疗药物诱导的原始卵泡激活过程中Kit/Kit Ligand 信号通路的影响。此外，分析外泌体中miRNA-2861的表达及其对化疗诱导原始卵泡激活过程中靶基因Kitlg的分子调控机制。最后，探讨人羊膜上皮细胞是否可作为一种载体通过传递miRNA-2861进一步提高修复卵巢功能的能力。

Chemotherapeutic agents are used to treat a wide variety of malignancies, and unavoidably cause progressive ovarian damage that can cause reduced fertility in young female patients.Previous studies have demonstrated that transplantation of hAEC or hAECs derived conditioned medium (hAECs-CM) could effectively alleviate chemotherapy-induced ovarian damage by inhibiting granulosa cells (GCs) apoptosis and promoting follicle development in animal models of premature ovarian failure and insufficiency (POF/POI). The mechanisms of benefit are known to be indirect, but the mediators have yet to be identified.Exosomes have emerged as a new avenue for cell-to-cell communication and an important component of paracrine pathway. A more interesting consideration is that stem cells could secrete exosomes, which play an important role in microRNA communication between donor cells and recipient tissues. In the current study, we mainly investigate the biological function of hAECs-derived exosomes (hAECs-exo) and whether they as a molecular basis regulate follicle activation induced by chemotherapy. In addition, we will detect the effect of exosomal miRNA-2861 derived from hAECs on follicle activation and analyze target gene of miRNA2861 in the process of follicle activation. Finally,our study will further discuss that exosomal miRNA-2861 derived from hAECs may offer a viable method for treating chemotherapy-induced ovarian injury.These novel insights offer a clue to the potential mechanism underlying hAECs promoting ovary function repair, which may be able to preserve the fertility in female cancer patients.