免疫抑制是临床化疗常见的不良反应，危害严重。“扶正固本”是中医治病的主要治则之一。党参、黄芪是常用的补益中药，在扶正中药临床配伍运用中具有高度代表性。临床研究表明：二者伍用对化疗后免疫抑制具有良好的调节作用，疗效显著。但二者干预免疫抑制的协同作用机制尚不明确。本项目拟采用UPLC-Q-TOF-MS/MS技术全面分析党参黄芪配伍血清药物化学，构建入血成分及代谢产物数据库；完善免疫功能调节相关靶点筛选数据库；采用网络药理学方法，构建“活性成分-作用靶点-免疫调节通路”网络图；采用免疫抑制动物模型验证党参黄芪配伍多靶点的协同作用机制，采用细胞实验分析活性成分与作用靶点间的相关性；以阐明党参-黄芪多成分、多靶点、多途径协同整合作用机制，为其临床伍用和活性部位开发提供科学依据，并进一步深化对中医“扶正固本”理论认识。

The immunosuppression is a common adverse reaction of chemotherapy in clinic. "Supporting the healthy energy" is one of the main therapeutic principle of traditional Chinese medicine (TCM) treatment. Codonopsis pilosula and radix astragali are two common tonic herb medicines, highly used as compatibility in TCM clinical application. Modern pharmacological studies have confirmed that the compatibility showed good immunoregulation effect on the immunosuppressive induced by chemotherapy. However, the mechanism of the compatibility is not clear so far. Therefore, serum pharmacochemistry was analyzed by UPLC-Q-TOF-MS/MS; immune regulating targets was improve based on our previous study; the network diagram of “active ingredients - targets - immune regulating pathways” was built based on network pharmacology; then, the integration mechanisms was confirmed using immunosuppressive animal model and the relationship between active ingredients and targets were studied by cell experiment. These results will clarify the mechanism of “multiple components, multiple targets and multiple approaches reaction” for the compatibility of codonopsis pilosula and radix astragali, provide a scientific basis for the usage in clinic and further development, and deepen the understanding of "Supporting the healthy energy "theory of TCM.