调节细胞粘附和细胞骨架的蛋白是上皮间质转化（EMT）的重要启动因子，是抑制肿瘤转移的靶点。我们已证实非小细胞肺癌（NSCLC）中异粘蛋白Metadherin（MTDH）调节细胞粘附和细胞骨架启动EMT，调控其表达的因子可能为抑制转移的突破点。长链非编码RNA（lncRNA）通过多种途径抑制编码蛋白基因表达发挥重要作用。我们发现反义lncRNA KB-1683C8与MTDH同位于8号染色体且序列不完全重叠，lncRNA芯片及qPCR均验证此lncRNA在NSCLC中表达增高，进一步证实外源性抑制该lncRNA可提高MTDH mRNA表达。因此我们提出：lncRNA KB-1683C8抑制MTDH从而启动EMT促进NSCLC转移。本研究将验证lncRNA KB-1683C8与MTDH的相关性，证实lncRNA、MTDH及EMT的关系，并探索其调节机制，为抑制NSCLC转移提供理论依据和基础。

The protein which regulates cell adhesion and cytoskeletal is an important initiation factor in epithelial to mesenchymal transition (EMT) and provides potential target for inhibiting metastasis. Our previous study found that the expression of Metadherin (MTDH) is significantly decreased in non-small cell lung cancer (NSCLC) and was correlated with lymph node metastasis. When the expression of MTDH exogenously decreased in NSCLC cell lines, cell morphology, cell skeleton and migratory ability were changed, suggesting that MTDH might be the initiating factor for EMT. The factor which controls MTDH expression may be the important breakthrough point for inhibiting metastasis in NSCLC. Long non-coding RNA (LncRNA) is demonstrated to inhibit the expression of protein coding gene through a variety of ways, playing an important role in gene expression. We identified an antisense lncRNA KB-1683C8 which located on chromosome 8, the same chromosome with MTDH and shared overlapped sequence with MTDH mRNA. The increased expression of lncRNA KB-1683C8 was revealed in three pairs of NSCLC tissues and corresponding normal tissues through lncRNA chip using RNA seq. Then it is verified by using realtime PCR that the expression of lncRNA KB-1683C8 is increased in NSCLC, and has correlation with the expression of MTDH. The expression of MTDH mRNA was increased when the expression of lncRNA KB-1683C8 was exogenously inhibited by siRNA transfection in NSCLC cells. Here, we give a hypothesis that lncRNA KB-1683C8 suppresses the expression of MTDH and therefore promotes the initiation of EMT. The aim of this research is to verify the relationship between the expressions of lncRNA KB-1683C8, MTDH and EMT, to explore the mechanism involved in the regulation of MTDH expression by lncRNA KB-1683C8, and to prove the correlation through the animal model, providing the basis and evidence for inhibiting metastasis in NSCLC.