RNA修饰是一个新兴的热门领域，转运RNA（tRNA）修饰是其中重要的组成部分。在酵母与人类中，tRNA甲基转移酶TRM6/61参与的起始tRNA m1A58甲基化修饰，对细胞活力的维持具有重要作用，但其在个体发育中的功能，尤其是植物生殖发育中的功能并不清楚。预实验结果显示：拟南芥trm61突变体早期胚胎与胚乳败育，且AtTRM61可同时结合AtTRM6与蛋白激酶AtMPK4，暗示AtTRM6/61为种子发育必需，且AtMPK4可能参与AtTRM61对起始tRNA修饰的调节。本项目将进一步研究AtTRM61的表达，利用胚胎与胚乳特异表达启动子获得部分回复的纯合突变体，阐明AtTRM61调控胚胎与胚乳发育的生物学功能；同时，通过酵母文库筛选与遗传互作分析等手段，探索AtTRM61调控起始tRNA表达与蛋白质翻译的分子网络机制。这将为解析tRNA修饰在植物生殖发育过程中的功能提供重要理论基础。

RNA modification is an emerging research area. The transfer RNA (tRNA) is an important component of RNA modification. In yeast and human cells, the m1A58 methylation modification of the initiated tRNA, which is carried out by AtTRM6/61, has been proved that it’s essential for cell viability. However, its functions in individual development are still unclear, especially in plant reproductive development. The previous results in this research showed that in trm61 mutants, there were severe defects in early embryo and endosperm development, and AtTRM61 can bind to AtTRM6 and the protein kinase AtMPK4 directly. These results suggested that the AtTRM6/61 complex is essential for seed development, and that AtMPK4 may work together with AtTRM61 in the regulation of initiated tRNA modification. Here, we will study the expression pattern of AtTRM61, and according to construct a partially complemental mutant using the tissue-specific promoters, we will further study the biological functions of AtTRM61 in the regulation of embryo and endosperm development. Moreover, by yeast library screening and genetic interaction assays, we will establish the molecular network of AtTRM61 regulated expression of initiated tRNA and the protein synthesis. This would shed light on the study of the functions of tRNA modification in plant reproductive development.