本研究拟采用生长因子基因修饰的肌腱干细胞（TSPCs），促进肌腱损伤愈合，同时降低粘连发生。首先，我们分离培养TSPCs并进行干细胞鉴定；接着，选取在肌腱愈合过程中发挥重要作用的两种生长bFGF和VEGF进行基因转染，构建质粒表达载体pEGFP-N1-bFGF、pEGFP-N1-VEGF并转染体外培养的TSPCs，制备bFGF或VEGF基因修饰TSPCs；最后，检测基因修饰TSPCs对离体、在体损伤肌腱愈合的调控作用，为过度到临床使用提供分子生物学及生物力学依据。本研究立足于过去十余年我们对肌腱损伤后一系列的细胞、分子机制研究，并延续了多年转基因治疗肌腱损伤的工作，同时整合了组织工程上最常使用的干细胞治疗方法，使得两者结合发挥双重效应，补充了肌腱愈合过程中急需的细胞和生长因子。本研究最终旨在将次探索成功的方法用于临床肌腱修复，以期解决手指肌腱愈合能力低这一难题。

This study aims to enhance the tendon healing and reduce adhesion formation by introduction of gene-modified tendon stem cells (TSPCs) in vivo and in vitro. Firstly, we isolate and culture the tendon stem cells from flexor tendons of chicken toes. Secondly, we chose the most important growth factors (bFGF and VEGF) in tendon healing to construct plasmid vectors, pEGFP-N1-bFGF、pEGFP-N1-VEGF, which are used to prepare the gene-modified TSPCs. Finally, we introduce the gene-modified TSPCs to the injured tendon and determine their effects on tendon healing. This study is based on a series of our investigations of cellular and molecular mechanism in tendon healing during the past ten years. It combines the gene therapy and tendon stem cells therapy, which would simultaneously supplement the injured tendons with cells and growth factors needed in the repair process. The ultimate purpose of this study is to explore the novel technique to resolve the clinical problem of tendon healing.