低频重复经颅磁刺激对皮质发育不良的形成及其所致癫痫发生的作用与机制研究

低频重复经颅磁刺激（rTMS）对癫痫发生的作用及机制仍不十分明确。先前的研究及课题组既往的研究基础提示低频rTMS有一定的抗癫痫作用，并可能通过GABAA受体发挥抑制癫痫发生的作用，而GABAA受体又与皮质发育不良的形成过程以及形成后致痫密切相关。本课题将采用冰冻损伤法制备皮质发育不良动物模型，通过杏仁核电点燃刺激研究其癫痫发生，应用MicroPET显现脑内GABAA受体的动态变化，采用膜片钳、免疫组化和免疫印迹等多种技术，探索低频rTMS对皮质发育不良的形成以及癫痫发生的作用，阐明GABAA受体在上述过程中的作用机制。本研究的完成将有助于我们进一步认识皮质发育不良的形成机理和致痫机制，明确低频rTMS干预癫痫发生的作用机理。本课题具有很大的科学特色和创新性, 为低频rTMS治疗皮质发育不良所致癫痫提供直接有力的理论依据。

The effect of low-frequency repetitive transcranial magnetic stimulation (rTMS) on epileptogenesis and its corresponding mechanism is still unclear. Previous investigations reported that low-frequency rTMS might have antiepileptic effect and play a role in epileptogenesis suppression mediated by gamma-aminobutyric acid (GABA) type A receptors, and GABAA receptors might be closely related to the formation of cortical dysplasia and cortical dysplasia-induced epileptogenesis. Herein, on the basis of freeze lesion-induced model and amygdala kindling method, this project aims to explore the effect of rTMS on epileptogenesis and clarify its detailed mechanism with involvement of GABA type A receptors with multiple methods, including MicroPET, patch clamp technique, immunohistochemistry, western blot and etc. This investigation would contribute to a deeper understanding of the formation of cortical dysplasia, its role in epilepsy, as well as the impacts of rTMS on epileptogenesis intervention, thus gives the technique a wider application in the clinical setting. This investigation, accordingly, enriched with scientific features and innovations, would potentially offer solid evidence on the inhibition effect of rTMS on cortical dysplasia-induced epilepsy.