MiR160d调控猕猴桃果实对灰霉病抗性的分子机制

猕猴桃是我国重要的经济林果之一。由灰葡萄孢菌引起的灰霉病给猕猴桃产业带来了巨大经济损失。抗病育种是防治猕猴桃灰霉病的有效措施，而剖析灰霉病应答的分子机制是猕猴桃抗性分子育种的理论基础。已有研究表明miR160通过IAA信号途径调控植物的抗病性。申请人前期研究发现miR160d参与猕猴桃果实灰霉病应答。基于此，本项目拟以遗传背景清晰的‘红阳’猕猴桃为研究对象，鉴定miR160d的靶基因，并利用过表达及VIGS技术解析其在猕猴桃灰霉病抗性中的功能；结合DNA pull-down、酵母单杂交等技术分析miR160d前体基因AcMIR160d与灰霉病应答相关蛋白的互作，确定调控miR160d的上游关键蛋白，阐明miR160d调控猕猴桃果实灰霉病抗性的分子机制。研究结果将从理论上丰富猕猴桃的抗病调控机制，也为实践中猕猴桃的抗病分子育种提供基因资源储备。

Kiwifruit is one of important economic fruits in China. Gray mold caused by Botrytis cinerea has brought huge economic losses to the kiwifruit industry. Disease resistance breeding is an effective measure to control kiwifruit gray mold, and elucidating the molecular mechanism responding to Botrytis cinerea is the theoretical basis for the resistance molecular breeding of kiwifruit. Studies have shown that miR160 regulates plant disease resistance through the IAA signaling pathway. Our previous study indicated that miR160d is involved in the response of kiwifruit to Botrytis cinerea. Here kiwifruit ‘Hongyang’ with clear genetic backgrounds is used as research objects. The present study includes identification of the target genes of miR160d and characterization of their function in kiwifruit gray mold resistance via over-expression and VIGS technologies; utilization of DNA pull-down and yeast one-hybrid technologies to analyze the interaction between miR160d precursor gene AcMIR160d and gray mold response related proteins. The results will theoretically enrich the disease resistance regulation mechanism of kiwifruit, and also provide gene resource reserves for the disease resistance molecular breeding of kiwifruit in practice.