内膜增生是动脉硬化闭塞以及动脉术后再狭窄的重要原因。增生的机制复杂，与血流动力学改变以及血管的顺应性有关，具体机制仍不明确。研究发现血管壁切应力（WSS）及血管壁环压（CS）通过H2O2-NADPH氧化酶(NOX)等途径引起内皮细胞和血管平滑肌细胞功能失调，从而启动内膜的增生，但具体过程仍需进一步研究。 本研究拟建立切应力及管壁环压两种血管壁受力模型，模拟病理学情况下血管壁剪切力，环压的变化，检测狭窄不同部位内膜增生厚度、内皮细胞功能、平滑肌细胞表型变化、炎症细胞浸润程度等之间差异，明确狭窄程度对内膜增生的影响；同时研究血流动力学异常相关NOX调控血管内皮细胞和平滑肌细胞的生物学功能变化；进而采用蛋白质组学研究方法，探索NOX相关更多调控蛋白，以补充和细化其调控通路，评估这种血流动力学异常与内膜增生的关系以及调控关键靶点。明确血流动力学调控机制，为进一步临床干预提控理论依据。

Atherosclerosis is a common disease in old people. Intimal hyperplasia is the important reason of the arteries occlusion and postoperative restenosis. Intimal hyperplasia mechanism is complex and not random. Intimal hyperplasia often appears at bifurcate artery and the place of vascular anastomosis. This is associated with hemodynamic change and vascular compliance, but the relationship between the degree of narrow and the stimulation of intimal hyperplasia is still not clear. Nowadays, study have found that the Wall shear stress (WSS) change affect the way such as H2O2-NADPH oxidase (NOX) to result to the cell function disorder, eventually lead to endometrial hyperplasia. Although H2O2 is defined as an ROS, unlike O2- it is not a free radical, in that it does not possess an unpaired electron in its outer shell. This renders H2O2 more stable and less reactive with other issue radicals and, thus, a more likely paracrine ROS. This study intends to establish animal artery narrow models and stent implantation models with the different degree, test the degree of narrow area, Wall shear tress, Circumferential strain, hyperplasia of endometrium thickness around the narrow area, smooth muscle cell phenotypic changes, the difference between degree of inflammatory cell infiltration, to clear the relationship between stenosis degree and endometrial hyperplasia, to clear the relationship between hemodynamic abnormalities with NOX biological function of vascular endothelial cells and smooth muscle cells. In addition, we will use the proteomics method to verify and find the new relevant mechanism of regulatory proteins, to develop other regulatory pathways and provide the theoretical and experimental basis for further clinical intervention.