先天样T细胞位于适应性和天然免疫系统之间，包括iNKT细胞和γδNKT细胞。它们具有效应者功能，能快速响应刺激而不需要抗原驱动的分化。PLZF基因对iNKT和γδNKT细胞发育和成熟是必须的，但它发挥功能的机制仍不完全清楚。本研究针对PLZF基因控制先天样T细胞效应者功能的现象，从先天样T细胞的胸腺滞留和迁移方面入手，拟采用新生小鼠胸腺移植模型和胸腺内FITC注射标记技术，确定PLZF基因控制先天样T细胞的胸腺滞留功能。通过PLZF基因缺失和转基因小鼠模型，结合流式细胞技术检测与T细胞迁移相关的蛋白表达，阐明PLZF基因调控先天样T细胞胸腺滞留的分子机制。研究结果揭示PLZF基因调控先天样T细胞发育的一种间接机制，即PLZF基因的表达使发育的胸腺细胞在胸腺滞留，使它们获得在胸腺内效应者成熟的时间，为PLZF这一多功能基因的作用机制增添了新的内容。

Innate-like T cells, including invariant Natural Killer T-cells (iNKT) and γδNKT cells, can be placed in-between the adaptive and innate immune systems.They have effector capabilities that allow them to rapidly respond to stimulation without the requirement for antigen-driven differentiation. PLZF gene is essential for the development and maturation of iNKT and γδNKT cells, however, the mechanisms that determine the fuction of PLZF is not completely understood. As PLZF gene controls the effector function of innate-like T cells, we firstly aim to investigate the thymic retention and migration of innate-like T cells. By using neonatal thymus transplantation model and intrathymic injection of FITC technique, the effect that PLZF gene controls the thymic retention of innate-like T cells will be confirmed. The expression of proteins associated with T cell migration will be detected by flow cytometry in PLZF deficient and transgenic mouse model, which will shed light on the molecular mechanisms by which PLZF regulates the thymic retention of innate-like T cells. These results will illustrate one indirect role of PLZF regulating the innate-like T cells development, which allows the thymic retention of developing thymocytes and provids time for their intrathymic effector maturation. This study will be helpful for understnding the mechanisms of PLZF gene, which has pleiotropic effects.