致病菌极易威胁人类健康，发展高灵敏检测方法对防控致病菌引起的公共卫生安全问题有重要意义。目前已有部分基于一体式脱氧核酶（DNAzyme）发展的致病菌检测方法，主要原理是结合目标致病菌分泌出的靶标物引起催化切割。但低浓度的目标致病菌导致靶标物浓度也低，此时游离的DNAzyme无法快速有效地结合靶标物，且有限的靶标物能激发的切割反应也有限，导致灵敏度大大受限。本项目拟结合同一目标致病菌的核酸适配体（aptamer）和DNAzyme，建立一种双功能核酸新检测体系，实现定向高效检测。同时，设计分体式DNAzyme（酶链和底物链），采用酶切循环放大策略，提高靶标物利用率。基于此，本项目将发展快速简单、高灵敏、强特异性的致病菌检测探针，并结合血糖仪开发便携检测方法，进行产品转化。后期将结合多孔纳米材料载药，设计功能核酸为智能开关，实现靶向检测同时给药杀菌，初步开展致病菌查杀一体化研究。

Development of sensitive detection methods is of great significance for preventing and controlling public health security caused by pathogenic bacteria, which pose a serious threat to human health. Several integrated DNAzyme-based probes have been developed for the detection of pathogenic bacteria, depending on the principle that combining with targets from pathogenic bacteria can causes catalytic cutting reaction. However, very small amount of bacteria produce a little targets so that free DNAzyme cannot combine with targets rapidly and efficiently. Limited number of targets just active limited amount of DNAzyme for catalytic cutting reaction. Therefore, integrated DNAzyme-based detection methods lead to difficulties in increasing sensitivity. To developing a novel detection system for targeted and high efficient detection, this project will combine pathogenic bacteria-specific aptamer and the same pathogenic bacteria-specific DNAzyme. Moreover, designing a cycle amplification strategy based on catalytic cutting reaction can be achieved by splited DNAzyme (including enzyme strand and substrate stand), resulting in improving utilization of targets from pathogenic bacteria. On this basis, simple, rapid, high sensitive and selective detection methods will be developed. Then, combining with glucometer to develop a simple detection method for daily monitoring, this method could be readily converted into a mature product. Furthermore, integrating porous nanomaterials for bactericide loading with functional nucleic acids as smart switches to control the drug release, this project will carry out the preliminary study of the integration of targeted detection and sterilization.