纳米材料易被植物吸收、累积，并扰乱植物的正常生理活性，进而对人体健康和环境造成潜在影响，因而受到广泛关注。目前对植物内源活性物质的分析主要采用破坏性采样方式，难以系统研究纳米材料对植物生理活性的影响，而活体采样技术可实时跟踪分析物在生物体中的浓度，更准确地反映植物体内活性物质的变化情况。本项目拟研制高选择性、高灵敏度的新型SPME探针，并发展低损伤性SPME原位活体采样分析方法用于内源植物激素的跟踪监测，进而系统研究纳米材料对植物生理活性的影响，探明纳米材料对内源植物激素的调控规律，并通过基因表达、DNA阶梯分析等手段研究其影响机制。本项目研究将有利于SPME原位与活体采样分析技术的进一步发展和应用，为生物体内源活性物质的监测提供新方法、新思路，也有助于更深入地认识纳米尺度物质的生物效应，对评价纳米材料的生态风险和健康风险具有重要的理论意义和现实意义。

Nanomaterials (NMs) are easily to be absorbed and accumulated by plants, then disturb the plant’s regular physiological activities, and rise wide concerns on the potential risks to the human health and environmental safety. Nowadays, the analysis of plant endogenous active substances is mainly conducted with destructive sampling methods, which are difficult to study the influence of NMs at plant’s regular physiological activities. By contrast, in vivo sampling methods can trace the concentrations of analytes in living organisms, and accurately reflect the variations of plant endogenous active substances under different stimuli. The present project is to develop novel solid phase microextraction (SPME) fibers with high selectivity and sensitivity to endogenous phytohormones, and then develop low destructive in vivo SPME sampling and analysis methods for the tracing of endogenous phytohormone in living plants in situ. Next, the influence of NMs on plants’ regular physiological activities will be systematically studied, and the influence mechanisms will be studied by gene expression and comet assay, etc. The present research could promote the development and application of in situ and in vivo SPME sampling and analytical methods, and provide new techniques and new strategies towards the monitoring of endogenous active substances in living organisms, which is significantly important for evaluating the eco-risks and human health risks of NMs.