苏云金芽孢杆菌Cry1Ab毒素对亚洲玉米螟ABC转运蛋白的分子互作研究

植物转苏云金芽孢杆菌（Bacillus thuringiensis 简称Bt）的大量种植使靶标害虫产生了抗性。ABC转运蛋白基因的突变和表达量的改变参与了多种鳞翅目害虫对Bt Cry1A毒素的抗性调节。本项目采用免疫组化的方法对其在玉米螟体内的表达进行组织定位，在证明ABC转运蛋白与Cry1Ab具有亲和力的基础上，运用RNAi和Crispr/Cas9技术验证其为Cry1Ab毒素的功能性受体蛋白, 明确ABC转运蛋白为Cry1Ab的的作用靶标；构建蛋白突变体后，测定Cry1Ab对表达ABC转运蛋白和突变体的S2细胞的活力变化，确定ABC转运蛋白与Cry1Ab的作用功能域。本研究将为亚洲玉米螟抗性机理奠定基础，也为延迟以ABC转运蛋白为抗性靶标的Cry毒素的抗性形成提供理论依据。

Transgenic plants producing Bacillus thuringiensis (Bt) toxins are threatened by insect resistance after massive planting. Mutation of ATP binding cassette transporter (ABC transporter protein) and its varied transcript are reported to be involved in Cry1A toxin resistance against various Lepidopteran strains. This study uses immunohistochemistry to reveal the localization of ABC transporter in Asian corn borer larvae; on the basis of clarifying ABC transporter binds Cry1Ab toxin with high affinity, we will analyze the functional role of ABC transporter as Cry1Ab binding receptor through RNAi and Crispr/Cas9 technologies, aiming to demonstrate that ABC transporter is the functional target of Cry1Ab; we will also testify the alteration of Cry1Ab toxicity on S2 cell with the expression of ABC transporter and mutation, and further analyze the functional region that ABC transporter interacts with Cry1Ab. This study will provide fundamental knowledge for different strategies to cope with Asian corn borer resistance, and also for delaying Cry resistance having ABC transporter as resistance target.