Hp感染与胃癌侵袭转移密切相关,但机制尚不明确.我们前期通过lncRNA芯片筛选发现lncRNA RCAN1在CagA+Hp感染的胃癌细胞中高表达,与胃癌转移密切相关;FISH提示lncRNA RCAN1与其亲本基因RUNX1 pre-mRNA存在共定位,干扰lncRNA RCAN1可以降低RUNX1 pre-mRNA稳定性;通过生物信息学预测,RIP,qRT-PCR实验还发现lncRNA RCAN1可以与PTBP1蛋白结合,共同促进RUNX1 pre-mRNA的稳定性.根据文献和前期实验,我们提出"lncRNA RCAN1与CagA+Hp感染密切相关,通过结合PTBP1增强RUNX1 pre-mRNA稳定性,促进RUNX1表达,介导胃癌转移"的假说,并拟通过RNA pull-down,Co-IP,质谱分析,分子生物学,细胞生物学等加以证实,为胃癌术后根除Hp提供新的理论依据和作用靶点.

Hp infection is closely related with metastasis of gastric cancer, but the mechanism is not clear. Our previous experiment found that expression of lncRNA RCAN1 is high in CagA+Hp infected gastric cancer cells through the lncRNA chip, which is closely related to the metastasis of gastric cancer; FISH indicated that lncRNA RCAN1 and RUNX1 pre-mRNA, its parent gene, are colocalized, interference of lncRNA RCAN1 can reduce the stability of RUNX1 pre-mRNA; Bioinformatics analysis, RIP and qRT-PCR also show that lncRNA RCAN1 can bind with PTBP1 protein, and promote the stability of RUNX1 pre-mRNA jointly. According to the literature and previous experiment, we put forward the hypothesis: " lncRNA RCAN1 is closely related to CagA+Hp infection, it can combine with PTBP1 to enhance stability of RUNX1 pre-mRNA, promote the expression of RUNX1, accelerate metastasis of gastric cancer". This hypothesis will be confirmed by RNA pull-down, Co-IP, mass spectrometry analysis,molecular biology, cell biology experiment in the further study. The confirmation of this hypothesis will provide a new theoretical basis and target for the eradication of Hp after surgery of gastric cancer.