异养反硝化脱氮的本质是反硝化菌利用分解碳源产生的还原力将硝酸盐、亚硝酸盐等还原为氮气，它在受污染水环境微生物治理及自然界氮循环中起重要作用。目前常用增加碳源的方法提高还原力产生，达到促进反硝化的目的。本项目研究了在不增加碳源的前提下，通过添加特定微生物同时促进异养反硝化脱氮过程的还原力产生与消耗，从而提高反硝化脱氮效率并减少反硝化中间产物积累的新方法。它通过对影响碳源分解产生还原力及硝酸盐转化消耗还原力的因素进行研究，获得添加的微生物种类及其促进反硝化菌还原力代谢的最佳条件，并从细胞形态、微生物关键分泌物、物质转运与胞内代谢、电子转移与传递等方面揭示其作用机理，最终通过实际反硝化系统的运行实现脱氮效率的显著提升及亚硝酸盐、一氧化氮和一氧化二氮等反硝化中间产物积累量大幅度减少。研究成果为增强异养反硝化脱氮提供新的途径，也为降低反硝化过程环境有害物质产生和促进自然界氮循环提供新的思路。

The essence of heterotrophic denitrification is that denitrifying bacteria reduce nitrate and nitrite to gaseous nitrogen by reducing power generated by decomposing carbon source. It plays an important role in polluted water environment biological denitrification and natural nitrogen cycle. At present, the method of increasing carbon source is commonly used to increase the reduction capacity, so as to promote denitrification. This project investigated a novel method to improve denitrification efficiency with the reduction of accumulation of denitrification intermediates by the supplement of specific microorganism without increasing carbon sources, while simultaneously promoting the production and consumption of reducing power in denitrifying bacteria metabolic process. The supplemented microbes and optimal conditions for the production and consumption of reducing power by microbial addition were obtained by studying the factors that affected the generation and consumption of reducing power involved respectively in carbon source decomposition and nitrate reduction. And the mechanism of microbial addition promoting the metabolism of reducing power was revealed from the aspects of cell morphology, key microbial secretions, substrate transport and intracellular metabolism, and electron transfer and transport. It remarkably improved the efficiency of real denitrification system, and reduced the accumulation of denitrification intermediates, such as nitrite, nitric oxide and nitrous oxide. The research results will provide a novel way for enhancing heterotrophic denitrification, and a new idea for reducing the generation of environmental harmful substances in denitrification process and promoting the natural nitrogen cycle.