小型一体化TERS仪器的研制及其在纳米表征中的应用

针尖增强拉曼光谱技术（TERS）因其能够提供分子指纹信息的纳米级空间分辨率和单分子检测灵敏度而日益受到关注。为了克服限制TERS广泛应用的商用仪器复杂性和实验室自制仪器稳定性不足问题，本项目提出研制一套高灵敏、高稳定性、小型一体化的TERS新仪器。通过电磁场模拟，获取最佳的针尖与基底耦合和激发与收集角度，指导TERS仪器的结构设计；利用课题组前期在便携拉曼仪器方面的积累，突破制约TERS小型化的关键瓶颈，设计集白光照明观察、激光激发、拉曼信号收集、检测和信号读出的一体化共焦光路，研制优异的电学噪声抑制的协同控制电路，发展针对TERS工作条件下的弱信号实时提取与快速成像算法及软件，开发针对TERS光路专用的针尖扫描头。以单层吸附分子和二维材料作为模型研究体系，在小型仪器上实现优于15nm的空间分辨率和单层分子的检测灵敏度，推动TERS技术走向实际应用，从而促进我国纳米尺度光谱学领域的发展。

Tip-enhanced Raman spectroscopy (TERS) has received increasing interest due to its nanometer spatial resolution and single molecule detection sensitivity. In order to overcome the limitations of complicated commercial instruments and instability of home-built instruments, this proposal aims at developing a new compact TERS instrument with high sensitivity and stability. The optimal tip-substrate coupling, excitation and collection configuration obtained by electromagnetic field simulation provides a practical guide to the design of the TERS system. On the basis of the previous research on portable Raman instruments, we will overcome the bottleneck in miniaturization of TERS instrument and design the confocal optical path with white light illumination and observation, laser excitation, and Raman signal connection, detection and readout. We will further develop the cooperative control circuit with an excellent electrical noise suppression and develop the algorithms and software for real-time extraction of weak signal and fast imaging under the TERS working conditions, and develop the dedicated tip scanning head to fit the TERS optical path. We will utilize adsorbed monolayer molecule and two dimensional materials as the model to realize the spatial resolution better than 15nm and monolayer detection sensitivity on the compact instruments. The success of this project will help push TERS to practical application and promote the rapid development of nano spectroscopy in China.