穴位敏化现象是近年研究的热点，是指在内脏疾病状态下，体表某些穴位可以发生感觉敏化和功能敏化。敏化穴位既是内脏疾病在体表的反应点，又是针灸特异性治疗内脏疾病的刺激点。目前对穴位敏化机制的研究还只是停留在内脏病变对体表敏化穴位组织化学特征的影响，而敏化穴位对病变内脏的调节效应及其机制还不清楚。本项目采用腹泻肠易激综合征（Diarrhea-predominant irritable bowel syndrome,D-IBS）内脏疾病模型，以内源性大麻素为切入点，采用形态学、行为学、电生理学、和组织化学方法研究体表敏化穴位对D-IBS大鼠内脏感觉和运动功能的调节效应及其机制，将穴位的治疗作用和穴位的状态联系起来，阐明穴位功能敏化的物质基础，为指导针灸治疗内脏疾病的临床选穴提供实验依据。

The phenomenon of acupoint sensitization is the hotspot now. Acupoint sensitization means visceral diseases can generate the sensitization of sensation and function of some acupoints in body surface. The sensitized acupoints are not only the responsive points of the visceral disorders in the body surface, but also the stimulus points of acupuncture on specific points treating visceral disorders. The past research on this phenomenon just focused on the influence of the visceral diseases on the histochemistry characteristics of the sensitized acupoints. The effects and the mechanism of the sensitized acupoints modulating visceral function are not identified. This project will establish diarrhea-predominant irritable bowel syndrome (D-IBS) model. Take endocannabinoids as penetration point to explore the effect and the mechanisms of sensitized acupoints in regulating visceral motility and sensation based on D-IBS model by the methods of morphology,etholog, electrophysiology, and histochemistry. We will to clarify the material basis of functional sensitization of acupoints by linking the therapeutic effects of acupoint to the state of of acupoints, and to provide experimental evidence for guiding selection of acupoints in treating visceral diseases in clinical.