CO2加富是目前我国设施栽培中大力推广的技术，不仅可以促进作物生产优化品质，也能够提高作物的抗病性。但是，CO2加富如何调控作物抗病性的分子机制尚未得到较好的阐释。我们前期研究中发现CO2加富条件下，番茄中重要的CO2捕获因子碳酸酐酶（βCA3）转录水平受细菌性叶斑病菌（Pst DC3000）诱导显著上调。本项目拟以番茄βCA3基因编辑和过表达植株为材料，探究βCA3基因在CO2加富环境诱导的细菌性叶斑病抗性中的功能；利用Thermoshift及显微观察等方法，明确βCA3介导的抗病性与气孔运动抗性途径和水杨酸抗性途径的关系；采用双分子互补和凝胶电泳迁移等方法筛选鉴定βCA3基因在转录水平和蛋白水平的调控因子。项目的开展有助于揭示番茄βCA3在CO2加富诱导设施作物细菌性叶斑病抗性中的作用及其调控机制，以期为我国设施园艺生产CO2 加富技术的推广应用及设施作物的安全高效生产提供理论基础。

In greenhouse, CO2 fertilization is widely utilized because it has been regarded as an effective approach for higher yield, better quality, modified defense of horticultural crop. However, the influence of CO2 fertilization on of plant pathogen defense response and its inner molecular mechanism are still not well characterized. Depend on our previous finding that one tomato CO2 sensor, a carbonic anhydrase (βCA3), was significantly induced by Pseudomonas syringae infection in CO2 enrichment condition. Therefore, βCA3 over-expression and CRISPR editing tomato plants are established for exploring the role of βCA3 in plant bacteria defense under CO2 enrichment. Depending on thermoshift assay and microscope observation, stomata- and salicylic acid-mediated defense signaling initiated by CO2 enrichment will be investigated how βCA3 participates in plant defense response. Besides, we will figure out how the pathogen-responded regulation factors transcriptional and posttranslational modulate βCA3 in pathogen defense signaling by Split-LUC and EMSA assays. These results will shed light on the systemic role of tomato βCA3 in CO2-initiated plant bacteria defense, and will be benefit on theoretical basis for CO2 fertilization in greenhouse horticultural crop production.