对一维纳米材料的液体分散和自组装行为的理解对发挥其性能优势和构筑纳米器件非常重要。埃洛石纳米管(HNTs)是新型的粘土类一维无机纳米材料，具有良好的水分散性和生物相容性，其有序结构的构建是研究者面临的共性难题，也是决定相关研究能否持续和开发其实际应用的关键问题。在前期研究的基础上，本项目提出了用受限挥发自组装法构筑HNTs有序表面并用于引导细胞取向生长的课题，将阐明HNTs尺寸结构、表面性质对其液体分散和组装行为的影响机制，研究其水分散液的液晶性质和流变性质的浓度、温度、力场等条件的依赖性。构筑不同受限挥发空间制备具有可控取向结构的HNTs有序表面，研究HNTs结构性质对形成有序结构的影响。阐明HNTs有序表面引导成纤维、成骨、成肌等细胞的取向生长的规律和机制。研究结果将为HNTs在可引导细胞取向生长的组织工程支架材料或细胞分选纳米器件等领域的应用奠定理论基础和实验依据。

Understanding the dispersion and self-assembly behavior of one-dimensional nanomaterials is very important for bringing their superiority into full play and building nanoscale devices. Halloysite nanotubes (HNTs), possing good water dispersion and excellent biocompatibility, is a new class of one-dimensional inorganic nanoclay material. Building the ordered structure of HNTs is the common problem of researchers, which also decides the persistence of the related research and is critical for developing their applications. On the basis of preliminary studies, the project proposes to build HNTs ordered surface via controlled evaporation self-assembly method for guiding the growth of cell orientation. The influence of size, structure, and surface properties of HNTs on the dispersion and assembly behavior will be studied. The dependence of liquid crystal properties and rheological properties of HNTs aqueous dispersions on the concentration, temperature, force field and other conditions will also illustrated. The effect of the surface properties of HNTs and the volatile limited space on the ordered structures will be investigated. The laws and mechanisms of HNTs ordered surface guiding fibroblasts, osteoblasts, myoblasts different cell epitaxy will be clarified. The results will lay the theoretical foundation and experimental evidence for HNTs application as material scaffolds in oriented cellar growth and cell sorting nanoscale devices.