乙烯调控水稻干旱胁迫反应的分子机制研究

目前关于植物激素参与其干旱胁迫反应的研究主要集中在ABA上，且多数是利用模式植物拟南芥进行的研究。水稻作为重要的单子叶模式植物，是重要的粮食作物，但乙烯信号转导在水稻干旱胁迫反应中的分子机制还不清楚。近年来我们分离鉴定了一系列的水稻乙烯反应突变体 (mhzs)，研究发现水稻乙烯信号途径既保守又有新机制。申请人发现水稻乙烯信号途径中的两个重要信号组分OsEIN2与OsEIL1的突变体具有抗旱的表型，RNA-seq技术初步分析显示：OsEIN2/MHZ7是乙烯调控水稻干旱胁迫反应的关键组分。我们将综合正向与反向遗传学，利用分子生物学、生物化学及细胞生物学等技术解析乙烯调控水稻干旱胁迫的分子机制及乙烯与ABA在水稻抗旱途径中的关系。该项研究有望解析乙烯调控水稻干旱胁迫反应的分子机制，将为改善水稻等作物对干旱胁迫的环境适应性提供重要的基因资源和理论及实践依据。

Current research on plant hormones involved in its response to drought stress focused on ABA, and most research is the use of the model plant Arabidopsis. Rice is an important model plant of monocotyledon and crop plant, but ethylene signaling transduction and the molecular mechanism of ethylene in rice environmental adaptability especially drought stress is still less known. During the studies of a series of ethylene-response mutants, we have found that the two mutants of Osein2/mhz7 and Oseil1/mhz6 shown drought tolerance. We will further analyze the differential genes among WT, Osein2/mhz7 and Oseil1/mhz6 via RNA-seq, use forward genetics and reverse genetics, and dissect the genes of mechanism of ethylene in rice drought resistance via molecular biology, cell biology and other technologies. This work should reveal the mechanism of ethylene negatively regulating drought stress in rice which is different from that in Arabidopsis, and should provide valuable gene resources of rice drought stress adaptation.