有毒植物所含成分复杂多样、毒性大、含量低且在人体内代谢迅速、中毒无特异性临床表现，由它们引发的的中毒案（事）件，在法庭科学领域一直是检验的难点。在血、脏器、消化物等复杂检材中，植物毒素经代谢分解，再加上杂质干扰，浓度极低甚至结构完全发生变化，现有的检验技术的方法灵敏度和准确度均难以达到办案要求。.为此，本课题拟以雷公藤、钩吻、夹竹桃为研究对象，通过动物实验，选用适当的前处理方法和技术设备，分析其所含成分及这些成分的理化性质和代谢产物，从中选取中毒标志物，建立复杂基质中这些标志物的检验方法。以此供案件承办单位对涉及有毒植物的凶杀案件、投毒案件、意外中毒事件等分析案件发生过程、印证犯罪过程等。为公安机关办案提供技术支撑和法庭证据，进而有力打击此类违法犯罪行为。

The toxins of poisonous plants are composed by a lot of ingredients.Their levels is low in human body and metabolize rapidly.Moreover there is no specific clinical manifestations in the cases which caused by plant toxins.So it's very difficult to identify these toxins in the field of forensic science.In complex samples such as blood,organs and gastric contents,plant toxins are breakdown through catabolism reactions. Because of serious matrix interferences and uncertain chemical structure,existing techniques and methods can not achieve the requirements of sensitivity and accuracy.. Therefore,this project is intended to choose three typical poisonous plants which is Tripterygium wilfordii Hook,Gelsemium elegans and Nerium oleander to study by animal experiments.Then find out appropriate pre-treatment methods and use advanced equipment such as LC/MS to analyse these toxins and their metabolites.We will choose some poisoning markers and establish the methods for detction these markers in complex matrices.In order to provide evidences and technical support when public security departments handle some poison cases and accidental poisoning cases involving poisonous plants.And fight against this kind of crime effectively.