调控材料表面性质使其能够抗补体激活并避免机体自身的免疫攻击是生物材料领域中的重要研究课题之一。细胞膜是自然界中最有效的血液相容表面，结合补体调控因子有望获得新型抗补体激活生物材料。然而由于磷脂的流动性，使仿细胞膜的磷脂双分子层表面在相关应用中受到诸多限制。本项目利用层层自组装技术和囊泡融合法，在聚电解质多层膜表面固载磷脂双分子层。通过多层膜和磷脂的偶联以及在磷脂中引入胆固醇，提高磷脂-基底和磷脂-磷脂的相互作用，从而增加磷脂膜的稳定性。在此基础上，在磷脂膜中引入生物素，通过生物素-亲和素作用实现补体调节因子在磷脂膜表面的高活性固定。研究上述材料的表面性能对蛋白黏附和补体系统激活的影响规律，进而阐明材料调控补体激活的主要因素。将上述材料置于全血中，检测材料在全血中的血液相容性，得到抗补体激活的血液相容性材料，为发展新型血液相容生物材料及其应用奠定基础。

One of the major problems in the field of biomaterials is to optimize their surface properties, so that the implants can inhibit the complement activation and then escape the innate immune system. The cell membrane is the best hemocompatible surface in nature. Binded the complement regulators,the membrance is expected to be the novel anticomplementary biomaterials. However, due to the fluidity and instability of lipids, the surfaces coated with mimetic phospholipid bilayers were restricted in the application. In this proposal, the phospholipid bilayers are immobilized on the polyelectrolyte multilayers via layer by layer technique and vesicle fusion method. To enhance the stability of the phospholipid bilayers, the phospholipids are conjugated to the multilayers and the cholesterols are inset between the phospholipids, which can improve the interactions of phospholipids - substrate and phospholipids - phospholipids, respectively. Moreover, the biotinyl-phospholipids are introduced to the bilayers, so that complement regulator can be bonded to the bilayers via biotin - avidin interactions and maintain their high biological activities. This study provides a versatile way to illustrate the influences of biomaterials properties on protein adhesion and complement activation, which is beneficial for us to elucidate the governing factors of biomaterials on the complement compatibility. Furthermore, the prepared surfaces are immersed into the blood and the hemocompatibility shall be investigated to obtain the anticomplementary hemocompatible biomaterials, which can present an intriguing avenue for selecting, designing and developing novel hemocompatible biomaterials in the research and therapy.