精子DNA损伤是导致不育的关键因素。环境污染物损伤精子DNA是主要原因之一。我们在前期研究中发现益肾通络方可明显修复由公认环境致癌污染物苯并(a)芘造成的精子DNA损伤。经lncRNA测序后发现其修复机制可能与lncRNA TCONS_00092730及减数分裂过程中关键基因MEI4密切相关，但具体机制不明。我们据此提出假说，益肾通络方可能通过调控lncRNA TCONS_00092730-MEI4通路降低减数分裂过程中的出错率，从而减少精子DNA碎片的产生。拟通过在生精细胞中过表达和沉默lncRNA TCONS_00092730和MEI4基因，以益肾通络方干预，采用相关实验技术检测lncRNA TCONS_00092730 - MEI4之间的相互作用；lncRNA相关多肽及MEI4蛋白在减数分裂生精细胞中的表达、定位；下游基因mRNA及蛋白的表达。揭示益肾通络方修复精子DNA损伤的机制。

Sperm DNA damage is a key factor results in infertility. Environmental pollutants damaging sperm DNA is one of the most important reasons. In our previous study, it was found that Yishen Tongluo Formula could significantly repair sperm DNA damage caused by the environmental carcinogenic pollutant benzo (a) pyrene. lncRNA sequencing revealed that its repair mechanism may be closely related to lncRNA TCONS_00092730 and MEI4, a key gene during meiosis, but the specific mechanism is unknown. Therefore, we hypothesized that Yishen Tongluo Formula may reduce the error rate during meiosis by regulating the lncRNA tcons_00092730-mei4 pathway, so as to reduce the production of sperm DNA fragments. lncRNA TCONS_00092730 and MEI4 genes were overexpressed and silenced in spermatogenic cells to interfere with Yishen Tongluo Formula. By applying relevant experimental techniques to detect the interaction between lncRNA tcons_00092730-mei4; Expression and localization of lncRNA-related polypeptides and MEI4 proteins in meiotic spermatogenic cells; mRNA and protein expression of downstream genes. To reveal the mechanism of Yishen Tongluo Formula to repair sperm DNA damage.