转移是食管癌死亡的主要原因。我们提出食管癌的核心病机为“胃脘干槁”，认为“甘润濡养”改善肿瘤微环境才有望阻断转移。M2型巨噬细胞是肿瘤微环境的重要组成部分，其分泌的IL-10刺激产生生长停滞特异性蛋白6（Gas6），Gas6介导的Gas6/Axl通路与转移密切相关。前期研究发现以“甘润濡养”立法的启膈方能抑制食管癌细胞中Gas6的表达，并通过抑制上皮细胞间质化及细胞骨架重构，抑制食管癌细胞迁移及侵袭。那么Gas6介导的Gas6/Axl通路在食管癌转移中发挥何作用？“甘润濡养”法是否通过调控巨噬细胞的极化而影响Gas6/Axl通路从而抑制转移？基于前期工作，我们提出科学假说“甘润濡养法通过改善食管癌微环境中巨噬细胞极化调控Gas6/Axl信号通路而抑制转移”。运用基因转染、免疫共沉淀、动物活体成像、微流控芯片等技术，以Gas6/Axl信号通路为主线系统揭示“甘润濡养”法抑制食管癌转移的机制。

Metastasis is the principal cause of death in patients with esophageal cancer. We believe the basic pathogenesis of the esophagus cancer is Yin deficiency of stomach. The metastasis will be blocked by the method of Ganrun Ruyang to improve the tumor microenvironment. The M2 macrophage is an important component of tumor microenvironment. IL-10 secreted by M2 macrophages stimulates the production of growth arrest-specific protein 6 (Gas6). The Gas6/Axl pathway which is caused by Gas6 is closely related to tumor metastasis. In the preliminary study, we found that Qigefang, which based on the method of Ganrun Ruyang , can obviously inhibit Gas6 in esophageal carcinoma cells. We found that Qigefang can inhibit the epithelial mesenchymal transition and the reconstruction of microfilament skeleton in esophageal cancer cells. Then the metastasis of esophageal cancer will be inhibit. What is the role of Gas6/Axl signaling pathway that is mediated by Gas6 in the transfer? Does "Ganrun Ruyang" method affect the Gas6/Axl pathway and inhibit metastasis by regulating the polarization of macrophages? Based on the previous work, we put forward the scientific hypothesis is that "Ganrun Ruyang" inhibits metastasis by improving macrophage polarization in esophageal cancer microenvironment and regulating Gas6/Axl Signaling pathway. Use gene transfection, immunocoprecipitation, animal imaging in vivo, microfluidic chip technology, to systematically discuss the mechanism of Ganrun Ruyang which inhibit the metastasis of esophageal cancer via Gas6/Axl pathway.