锌指蛋白在植物生长发育及逆境应答过程中起重要的调控作用，但对其作用机理知之甚少。我们前期研究发现:玉米锌指蛋白ZmLSD1是直接调控耐盐基因ZmLTP表达的转录因子；过量表达ZmLSD1可提高转基因拟南芥的耐盐性；ZmPti1可以结合并磷酸化ZmLSD1。推测ZmLSD1的转录活性及生物学功能可能被ZmPti1的磷酸化所调节。为证实此设想，拟利用超表达和基因编辑技术获得转ZmLSD1基因玉米，明确ZmLSD1在玉米中的耐盐功能；检测并点突变ZmPti1对ZmLSD1的磷酸化位点，以野生型及点突变蛋白为对象，分析ZmLSD1的磷酸化是否影响其与启动子的结合能力、转录活性及生物学功能。同时，以野生型、超表达和基因编辑的转基因玉米为材料，利用ChIP-seq、RNA-seq等技术，解析ZmLSD1的下游调控网络。研究结果对ZmLSD1耐盐机理的阐明以及玉米的改良具有重要意义。

Zinc finger proteins play important roles in regulation of plant development and responses to stresses. But their regulation mechanisms were few reported. In our previous studies, ZmLSD1, a zinc finger protein is a transcription factor regulating the expression of ZmLTP3. Overexpression of ZmLSD1 enhanced the tolerance to salt in Arabidopsis. ZmPti1 can interact with and phosphorylate ZmLSD1, suggesting that the activities and functions of ZmLSD1 could be regulated by the phosphorylation of ZmPti1. In this study, overexpression and gene editing methods will be used to transform maize. The functions in stresses resistance of ZmLTP3 will be analyzed in transgenic maize.The phosphorylation site of ZmPti1 to ZmLSD1 will be checked and site-directed mutated, so as to mutational proteins. The effects of different phosphorylation levels of ZmLSD1 on its DNA-binding affinity, ability of transcription and the stresses tolerance will be analyzed. Furthermore, to explore other genes regulated by ZmLSD1, the wild type, overexpression and gene editing transgenic maize lines will be used for analysis by ChIP-PCR and RNA-Seq. The results will explain the function and mechanism of ZmLSD1, and provide theoretic support and new material for abiotic tolerant maize breeding.