结肠癌是人类主要恶性肿瘤之一，寻找有效的预后指标和治疗靶点对结肠癌的治疗具有重要意义。GOLGA8B基因在结肠癌组织中过表达，且与患者较差的预后相关。但其调控机制及在结肠癌发展中的作用，尚不清楚。前期研究发现，非编码区SNP rs6495690位点的A/C多态性与GOLGA8B的表达显著相关，rs6495690可能作为等位特异性增强子调控GOLGA8B的表达。本项目将首先明确rs6495690对GOLGA8B表达的影响，揭示rs6495690等位特异性调控GOLGA8B表达的机制，通过功能实验明确GOLGA8B在结肠癌发展中的作用。此外，通过肿瘤流行病学调研探究rs6495690不同基因型与结肠癌患病风险之间的关系。通过本研究，拟阐明遗传变异（rs6495690）影响结肠癌发展进程的机制，为以GOLGA8B为靶标的结肠癌干预策略提供思路。

Colon cancer is one of the main malignant tumors in human beings. It is of great significance to find effective prognostic indicators and therapeutic targets for the treatment of colon cancer. The GOLGA8B(Golgin A8 family member B) gene is found to be overexpressed in colon tumor tissues and closely related to the poor prognosis in patients. However, its regulatory mechanism and the role in the development of colon cancer are still unkown. Our previous studies found that the A/C polymorphism in the non-coding SNP rs6495690 locus was significantly correlated with the expression of GOLGA8B, and rs6495690 may act as an allele-specific enhancer to regulate the expression of GOLGA8B. This project will confirm the effect of rs6495690 on GOLGA8B expression, and reveal the mechanism of GOLGA8B expression regulation by rs6495690, as well as unravel the role of GOLGA8B in colon cancer through a series of functional experiments. In addition, the relationship between rs6495690 genotypes and the risk of colon cancer will be investigated through cancer epidemiology research. Our project aims to elucidate the mechanism of genetic variation (rs6495690) affecting colon cancer progression, laying the foundation for developing intervention strategies targeting GOLGA8B.