microRNA是调控胰岛素敏感性的重要因子，在肥胖机体中表达异常。前期研究提示：microRNA-450a-5p在肥胖和T2DM个体中显著高表达，生物信息学预测其可调控DUSP10。 DUSP10可通过调节JNK的磷酸化，参与胰岛素抵抗（insulin resistance, IR）和T2DM的发生过程。提出假设：肥胖状态下，脂肪组织源性microRNA-450a-5p表达升高，通过靶向下调肝脏及脂肪组织DUSP10的表达，促进JNK磷酸化激活，引起下游信号转导过程发生改变，诱发 IR，最终导致T2DM。为此，本研究在检测不同状态个体血清样本microRNA-450a-5p表达水平的基础上，分析其与受试个体一般资料及生化指标的相关性。在体外细胞培养及构建动物模型的基础上，明确microRNA-450a-5p是否通过下调DUSP10表达导致IR和T2DM的发生，并阐明其可能的分子机制。

MicroRNA is a critical factor in regulating insulin sensitivity and abnormal expression in obese organisms. Previous studies suggest that microRNA-450a-5p is high expression in obesity and T2DM organisms, and bioinformatic predicts that it can regulate DUSP10 which can participate in insulin resistance and T2DM by regulating the phosphorylation of JNK. It is hypothesized that the expression of adipose tissue-derived microRNA-450a-5p is increased in obese state, and down-regulated the expression of DUSP10 in liver and adipose tissue, which promotes the activation of JNK phosphorylation, changes the downstream signal transduction process, and induces IR, eventually led to T2DM. Therefore, on the basis of detecting the level of microRNA-450a-5p expression in serum samples of different individuals in different states, the correlation between the expression level and the general data and biochemical indexes of the subjects was analyzed in this study. On the basis of cell culture in vitro and establishment of animal models, to determine whether microRNA-450a-5p is responsible for IR and T2DM by down-regulating the expression of DUSP10, and clarify its possible molecular mechanism.