病毒的寄生性，使开发高选择性的抗病毒药物存在极大困难，至今仍未取得重大突破。植物中存在丰富的天然化合物库，从植物中开发高效、低毒、环境友好的抗病毒剂是目前的研究热点。前期的研究中发现紫茎泽兰的甲醇提取物中存在具有显著钝化烟草花叶病毒，抑制病毒侵染和增殖的活性成分。本项目旨在前期研究的基础上，通过活性跟踪，运用HPLC-MS,HPLC-NMR等现代化的分离纯化技术和结构鉴定手段，从锁定的活性部位，分离富集具有抗病毒活性的目标化合物，研究其结构和活性特点，分析构效关系，并研究其对HBV，HCV，HIV等人类疾病相关病毒的抑制活性，从中寻找高效、低毒的天然抗病毒先导化合物，并结合生物化学和分子生物学手段进一步研究其抗病毒机理，为新型植物病毒抑制剂的研制提供科学依据。同时也为今后有综合治理及有效利用入侵植物紫茎泽兰起到积极的推动和指导作用。

It is difficult to develop safe and effective antiviral drugs because viruses use the host's cells to replicate，and until now the great breakthrough in this research field is still blank. Natural products from plants have been proved to be a rich resource because plants have already evolved multiple mechanisms to selectively suppress pathogens by production of secondary metabolites with antimicrobial activities. The great pool of natural products provided a lot of opportunity for researchers to develop virus inhibitors with high efficiency and low toxicity from plants. Previously we found that the methanol extract of Eupatorium adenophorum Spreng showed dramatic antiviral activity to tobacco mosaic virus (TMV), it was proved that some components of the extract have the obvious function of inhibiting virus infection and replication. In this project, we will clarify the structures of the objective components responsible for the anti-TMV activity according to bioassay-guided isolation of the related extract by methods of modern purification and structure-identification techniques such as HPLC-MS, HPLC-NMR. In addition to the structures study of the antiviral components we will analyze the structure-activity relationship of the active compounds, and we will also test the inhibiting activity of the compounds to several human viruses such as HIV and hepatitis B and C. To seek for effective antiviral lead compounds with low toxicity we will further study the antiviral mechanisms of those antiviral compounds from E. adenophorum Spreng by methods of biochemistry and molecular biology. Those works will provide a base for further exploration of new type of plant virus inhibitors. In addition, our work will promote the utilization and management of the invasive plant E. adenophorum Spreng.