周围神经再生中心问题是再生轴突对选择特定运动感觉传导通路精准诱向性生长，不同类型神经元功能性突触连接重建。课题组前期研究发现，跨突触信号分子Neurexin-Neuroligin特异性调控运动感觉神经纤维发育与再生过程。其中，Neuroligin-1特异性促运动神经元突触重塑；Neuroligin-2特异性促感觉神经元突触重塑。并且发现不同解剖部位施万细胞和成纤维细胞，亦具有各自独特的运动感觉特异表型。本研究通过结构/微环境双重仿生，3D打印含运动感觉特异细胞Y型丝素/明胶支架，内置Neurexin-Neuroligin缓释微球丝素悬丝，构建运动感觉诱导型组织工程化神经。以期通过再生早期阶段干预，达到有利于运动感觉神经纤维正确有序性生长，提高神经再生速度和效率，实现对靶器官更精准更有效重支配。本研究为更全面地理解周围神经再生中运动感觉神经再生与功能重建，以及临床修复周围神经损伤提供新线索。

A central issue in peripheral nerve regeneration is that the regenerative chemotactic axons to select their predictable motor-sensory pathways and that the manner in which various neurons reestablish their distinct phenotypes by forming functionally appropriate synaptic connections. Our previous study indicated that the transsynaptic signals Neurexin-Neuroligin specifically regulated the motor and sensory neural development and regeneration. Neuroligin-1 specifically promotes motor neuron synaptic rearrangement, while, Neuroligin-2 specifically promotes sensory neuron synaptic rearrangement. Moreover, we found that Schwann cells and fibroblasts dissected from different anatomical regions also have their distinct motor and sensory phenotypes. Based on this premise, this project aims to construct a tissue engineered nerve grafts composed of a controlled release Neurexin-Neuroligin microsphere which crosslinked with silk fibroin and a 3D printing of silk/gelatin-based scaffold which integrated with motor-sensory phenotype Schwann cells and fibroblasts. Through optimizing the specific anatomical microenvironment in the early regenerative processes, our design is hoped to facilitate the appropriate selection of motor and sensory pathways, promote the efficiency of neurites outgrowth, and thus advance the efficiency of reinnervation of distal target organs. This study provides new insights into the motor and sensory functional recovery during peripheral nerve regeneration, and also contributing to the development of a potential therapeutic target for peripheral nerve injury.