茶树菇pebp基因在子实体发育中的作用分析

磷脂酰乙醇胺结合蛋白(PEBP)家族广泛存于在生物体中，参与调节动植物多种重要的生理活动，如植物营养生长到生殖生长的转变，然而真菌中尚未对其功能进行阐释。本研究拟以获得的茶树菇AC0007菌株子实体特异表达基因Acpebp1、Acpebp2-1和Acpebp2-2-1为研究对象，分析其在不同发育时期和不同组织中的表达谱，推测基因表达模式与发育行为的相关性；构建三个pebp基因过表达/反义抑制转化菌株，明确基因在菌株营养生长到生殖生长转变和子实体发育过程中的作用；构建野生型和过表达/反义抑制菌株转录组，转录组集成分析pebp基因组可能的关联基因，并映射到对应的分子途径，推测pebp可能参与的调节路径；从基因表达模式、基因功能分析和分子途径探索三个层面，深入理解PEBP家族基因在茶树菇子实体发育中的作用，为担子菌其它种类的生殖遗传研究提供借鉴。

Phosphatidylethanolamine-binding protein (PEBP) family widely present in living organisms, which regulate many kinds of physiological activities such as the transition of vegetation to reproduction growth of plant, but the functions of them in fungi remain unknown. In this study, three genes, Acpebp1, Acpebp2-1 and Acpebp2-2-1 expressed specially in fruiting bodies of Agrcobye aegerita AC0007, were investigated on the expression pattern of them in different development stages and tissues for verifying the relationship of developmental behavior and gene expression pattern; The constructions of overexpression/RNAi transformation strains were carried out for verifying the effect of three pebp genes on the transition of vegetation to reproduction growth and the configuration characteristic of fruiting bodies; The integration analysis was conducted to find the relevant genes with pebp based on the transcriptome, and they were mapped to the corresponding molecular pathways for exploring the regulating pathway which pebp gene involved; three levels of gene expression pattern, gene function analysis and the exploration of molecular pathway were conducted to study the effect of PEBP family on the fruiting body development of Agrocybe aegerita, and provides insight for reproduction genetics of other basidiomycetes.