骨诱导性生物材料在骨缺损修复中具有切实可行的应用前景，对材料的优化及相关机制研究是其广泛应用的基础。前期研究表明骨诱导性材料植入体内后可促进破骨细胞分化；而材料的微结构可能在此过程中通过对细胞粘附及细胞骨架的调节而影响破骨细胞的分化成熟；Rho GTPases作为细胞骨架的关键调控因子，其是否在此过程中发挥作用尚不清楚。本研究通过对反应条件的控制形成具有骨诱导性的磷酸三钙陶瓷材料，与小鼠前破骨细胞株RAW264.7复合培养，利用TRAP染色、细胞骨架蛋白和粘附相关蛋白的荧光染色、吸收陷窝的扫描电镜分析以及qRT-PCR系统地评价破骨细胞在骨诱导材料中分化成熟的动态变化；通过引入Rac1及其抑制剂探索骨诱导材料对破骨细胞分化成熟的作用是否受到Rho GTPases的调控，该研究有助于完善骨诱导相关生物学机制，为进一步优化材料奠定基础。

The study on the mechanism of osteoinductive materials is very important for the optimization of materials, which is the basis for its wide application. Previous studies demonstrated the osteoinductive materials can promote the osteoclastogenic differentiation of monocyte in vivo, and the microstructures on the materials believed to be the most important contributing factor, by affecting the arrangement of cytoskeleton and focal adhesions, finally determine the differentiation of cells. However, as one of the most important factors in cytoskeleton dynamics, whether the osteoclastogenic differentiation of monocyte on the microstructures controlled by Rho GTPases has not been clarified. In this study, two types of tricalcium phosphate ceramic disc with submicron or micron topography would be used, the murine RAW264.7 macrophages would be cultured on the surface of each discs for up to 5 d in the presence of osteoclast differentiation factor RANKL. TRAP staining, and Fluorescent staining for F-actin, vinculin and Hoechst would be used to evaluate the osteoclast differentiation of cells, meanwhile the qRT- PCR would be used to evaluate the markers for osteoclastogenesis, after 21 day of cell culture the resorption lacuna formation would be evaluate by SEM. Finally, the Rac1 and Rac1 inhibitors would be used to clarify whether the osteoinductive materials induced osteoclastogenesis could be regulated by Rho GTPases. This study would help us clarify how the osteoinductive material affect the osteoclast differentiation and further elucidate the biological mechanisms of osteoinduction, which provide the basis for further optimization the osteoinductive materials.