翠绿针毛蕨是一种具有显著抗肿瘤活性的土家族药用蕨类植物，该植物富含结构新颖罕见的环己烯酮色原酮类成分，前期已初步确认环己烯酮色原酮提取物为其抗肿瘤活性部位，但具体药效物质基础尚不清楚。本项目基于“体内代谢过程的药效成分识别”的新策略，借助网络药理学分析手段，建立“化学成分-序贯代谢-网络靶标”三联研究模式，阐明翠绿针毛蕨抗肿瘤作用的药效物质基础，明确环己烯酮色原酮的体内代谢行为规律，探讨这类化合物抗肿瘤作用的分子机制，为其今后开发应用提供科学依据。

Macrothelypteris viridifrons (Tagawa) Ching is a medicinal fern for Tujia people, which shows highly strong anti-tumor activity. Previous study showed that the active extract was rich of novel cyclohexenonechromones, but the active constituents are not clear. In the study, based on metabolism analysis in vivo and network pharmacology, the strategy of “Chemical Constituents-Sequential Metabolism-Network targets” was established to elucidate the active constituents of Macrothelypteris viridifrons for the anti-tumor effect and clarify the metabolic patterns and pharmacological mechanism of cyclohexenonechromones, which can provide scientific basis for the further development.